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10.3390/ijms17050780 http://scihub22266oqcxt.onion/10.3390/ijms17050780 27213360!4881597!27213360     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2016 ; 17 (5): ä Nephropedia Template TP {{tp|p=27213360|t=2016. Angiotensin II Stimulation of DPP4 Activity Regulates Megalin in the Proximal Tubules |pdf=|usr=}}{{27213360}} gab.com Text Angiotensin II Stimulation of DPP4 Activity Regulates Megalin in the Proximal Tubules JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=27213360 #FOAvip Proteinuria is a marker of incipient kidney injury in many disorders, including obesity. Previously, we demonstrated that megalin, a receptor endocytotic protein in the proximal tubule, is downregulated in obese mice, which was prevented by inhibition of dipeptidyl protease 4 (DPP4). Obesity is thought to be associated with upregulation of intra-renal angiotensin II (Ang II) signaling via the Ang II Type 1 receptor (AT(1)R) and Ang II suppresses megalin expression in proximal tubule cells in vitro. Therefore, we tested the hypothesis that Ang II will suppress megalin protein via activation of DPP4. We used Ang II (200 ng/kg/min) infusion in mice and Ang II (10(-8) M) treatment of T35OK-AT(1)R proximal tubule cells to test our hypothesis. Ang II-infused mouse kidneys displayed increases in DPP4 activity and decreases in megalin. In proximal tubule cells, Ang II stimulated DPP4 activity concurrent with suppression of megalin. MK0626, a DPP4 inhibitor, partially restored megalin expression similar to U0126, a mitogen activated protein kinase (MAPK)/extracellular regulated kinase (ERK) kinase kinase (MEK) 1/2 inhibitor and AG1478, an epidermal growth factor receptor (EGFR) inhibitor. Similarly, Ang II-induced ERK phosphorylation was suppressed with MK0626 and Ang II-induced DPP4 activity was suppressed by U0126. Therefore, our study reveals a cross talk between AT(1)R signaling and DPP4 activation in the regulation of megalin and underscores the significance of targeting DPP4 in the prevention of obesity related kidney injury progression. Twit Text FOAVip Angiotensin II Stimulation of DPP4 Activity Regulates Megalin in the Proximal Tubules ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=27213360 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27213360 #FOAvip English Wikipedia <ref>{{Cite pmid|27213360}}</ref> Angiotensin II Stimulation of DPP4 Activity Regulates Megalin in the Proximal Tubules #MMPMID27213360 Aroor A; Zuberek M; Duta C; Meuth A; Sowers JR; Whaley-Connell A; Nistala R Int J Mol Sci 2016[May]; 17 (5): ä PMID27213360show ga Proteinuria is a marker of incipient kidney injury in many disorders, including obesity. Previously, we demonstrated that megalin, a receptor endocytotic protein in the proximal tubule, is downregulated in obese mice, which was prevented by inhibition of dipeptidyl protease 4 (DPP4). Obesity is thought to be associated with upregulation of intra-renal angiotensin II (Ang II) signaling via the Ang II Type 1 receptor (AT(1)R) and Ang II suppresses megalin expression in proximal tubule cells in vitro. Therefore, we tested the hypothesis that Ang II will suppress megalin protein via activation of DPP4. We used Ang II (200 ng/kg/min) infusion in mice and Ang II (10(-8) M) treatment of T35OK-AT(1)R proximal tubule cells to test our hypothesis. Ang II-infused mouse kidneys displayed increases in DPP4 activity and decreases in megalin. In proximal tubule cells, Ang II stimulated DPP4 activity concurrent with suppression of megalin. MK0626, a DPP4 inhibitor, partially restored megalin expression similar to U0126, a mitogen activated protein kinase (MAPK)/extracellular regulated kinase (ERK) kinase kinase (MEK) 1/2 inhibitor and AG1478, an epidermal growth factor receptor (EGFR) inhibitor. Similarly, Ang II-induced ERK phosphorylation was suppressed with MK0626 and Ang II-induced DPP4 activity was suppressed by U0126. Therefore, our study reveals a cross talk between AT(1)R signaling and DPP4 activation in the regulation of megalin and underscores the significance of targeting DPP4 in the prevention of obesity related kidney injury progression. |*Gene Expression Regulation[MESH] |*MAP Kinase Signaling System[MESH] |Angiotensin II/*metabolism/pharmacology[MESH] |Animals[MESH] |Cell Line[MESH] |Dipeptidyl Peptidase 4/*metabolism[MESH] |Enzyme Inhibitors/pharmacology[MESH] |Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors/metabolism[MESH] |Kidney Tubules, Proximal/*metabolism[MESH] |Low Density Lipoprotein Receptor-Related Protein-2/*biosynthesis[MESH] |Male[MESH] |Mice[MESH] |Mice, Obese[MESH] |Obesity/complications/metabolism[MESH]Angiotensin II Stimulation of DPP4 Activity Regulates Megalin in the P(epmc).pdf DeepDyve Pubget Overpricing