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10.1007/s00253-016-7542-4 http://scihub22266oqcxt.onion/10.1007/s00253-016-7542-4 27129532/?report=reader!4947482!27129532     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Appl+Microbiol+Biotechnol 2016 ; 100 (16): 7181-92 Nephropedia Template TP {{tp|p=27129532|t=2016. Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells |pdf=|usr=}}{{27129532}} gab.com Text Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells JO: Appl Microbiol Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=27129532 #FOAvip Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. Twit Text FOAVip Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells ????Appl Microbiol Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=27129532 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27129532 #FOAvip English Wikipedia <ref>{{Cite pmid|27129532}}</ref> Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells #MMPMID27129532 Frensing T; Kupke SY; Bachmann M; Fritzsche S; Gallo-Ramirez LE; Reichl U Appl Microbiol Biotechnol 2016[Aug]; 100 (16): 7181-92 PMID27129532show ga Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. |*Virus Replication[MESH] |Animals[MESH] |Cell Line[MESH] |Dogs[MESH] |Humans[MESH] |Influenza A Virus, H1N1 Subtype/genetics/*growth & development[MESH] |Influenza Vaccines/*biosynthesis/immunology[MESH] |Influenza, Human/immunology/*prevention & control[MESH] |Madin Darby Canine Kidney Cells[MESH] |RNA, Viral/*biosynthesis[MESH]Influenza virus intracellular replication dynamics, release kinetics, (epmc).pdf DeepDyve Pubget Overpricing