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10.1002/bab.1498 http://scihub22266oqcxt.onion/10.1002/bab.1498 27067406!7161787!27067406     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechnol+Appl+Biochem 2017 ; 64 (3): 443-448 Nephropedia Template TP {{tp|p=27067406|t=2017. Development of a degenerated TaqMan real-time Q-PCR for detection of bacteria-free DNA in dialysis fluid |pdf=|usr=}}{{27067406}} gab.com Text Development of a degenerated TaqMan real-time Q-PCR for detection of bacteria-free DNA in dialysis fluid JO: Biotechnol Appl Biochem http://www.kidney.de/pget.php?&tw=1&pmid=27067406 #FOAvip Bacterial-derived DNA fragments (BDNAs) have been shown to be present in a dialysis fluid, to pass through dialyzer membranes, and to induce interleukin 6 (IL-6) in mononuclear cells. DNA fragments are thought to be derived from microorganisms inhabiting hemodialysis water and fluid. The primary aim of the present study was to develop two degenerated TaqMan real-time quantitative-PCR (Q-PCR) for detection of a broad range of bacterial DNA that specifically detect 16S ribosomal DNA (rDNA) (862 and 241 bp) and evaluate the efficiency of the Bellco Selecta resin to capture the BDNAs in the dialysis fluid. For this purpose, we decided to compare measurements of unfragmented samples (9.8 x 10(5) Escherichia coli genome) with artificially fragmented DNA samples. We assessed two broad-range real-time PCR targeting bacterial 16S rRNA genes for detection of fragmented and unfragmented bacterial DNA in the dialytic fluid and demonstrated that Bellco Selecta resin is capable of retaining these types of bacterial DNA. Twit Text FOAVip Development of a degenerated TaqMan real-time Q-PCR for detection of bacteria-free DNA in dialysis fluid ????Biotechnol Appl Biochem http://www.kidney.de/pget.php?&tw=1&pmid=27067406 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27067406 #FOAvip English Wikipedia <ref>{{Cite pmid|27067406}}</ref> Development of a degenerated TaqMan real-time Q-PCR for detection of bacteria-free DNA in dialysis fluid #MMPMID27067406 Bergallo M; Loiacono E; Rizzo G; Caiazzo M; Palladino G; Amore A Biotechnol Appl Biochem 2017[May]; 64 (3): 443-448 PMID27067406show ga Bacterial-derived DNA fragments (BDNAs) have been shown to be present in a dialysis fluid, to pass through dialyzer membranes, and to induce interleukin 6 (IL-6) in mononuclear cells. DNA fragments are thought to be derived from microorganisms inhabiting hemodialysis water and fluid. The primary aim of the present study was to develop two degenerated TaqMan real-time quantitative-PCR (Q-PCR) for detection of a broad range of bacterial DNA that specifically detect 16S ribosomal DNA (rDNA) (862 and 241 bp) and evaluate the efficiency of the Bellco Selecta resin to capture the BDNAs in the dialysis fluid. For this purpose, we decided to compare measurements of unfragmented samples (9.8 x 10(5) Escherichia coli genome) with artificially fragmented DNA samples. We assessed two broad-range real-time PCR targeting bacterial 16S rRNA genes for detection of fragmented and unfragmented bacterial DNA in the dialytic fluid and demonstrated that Bellco Selecta resin is capable of retaining these types of bacterial DNA. |*Genes, rRNA[MESH] |DNA, Bacterial/*genetics[MESH] |DNA, Ribosomal/*genetics[MESH] |Escherichia coli/*genetics[MESH] |RNA, Ribosomal, 16S/*genetics[MESH]Development of a degenerated TaqMan real-time Q-PCR for detection of (epmc).pdf DeepDyve Pubget Overpricing