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10.1007/s10157-015-1214-z http://scihub22266oqcxt.onion/10.1007/s10157-015-1214-z 26715508!ä!26715508 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Exp+Nephrol 2016 ; 20 (5): 679-688 Nephropedia Template TP {{tp|p=26715508|t=2016. NF-kappaB-dependent increase in tissue factor expression is responsible for hypoxic podocyte injury |pdf=|usr=}}{{26715508}} gab.com Text NF-kappaB-dependent increase in tissue factor expression is responsible for hypoxic podocyte injury JO: Clin Exp Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=26715508 #FOAvip BACKGROUND: Fibrin deposition within glomeruli is commonly seen in kidney biopsy specimens, suggesting enhanced coagulant activity. Tissue factor (TF) is a coagulation factor which is also related to various biological effects, and TF is upregulated by hypoxia in cancer cells. Recently, hypoxic podocyte injury has been proposed, therefore, we investigated TF expression in hypoxia. METHODS: Conditionally immortalized human podocytes were differentiated and treated under hypoxic or normoxic conditions. mRNA expressions of TF and tissue factor pathway inhibitor (TFPI) were analyzed by quantitative RT-PCR. Protein levels of TF and TFPI were tested by enzyme-linked immunosorbent assay. We employed small interfering RNA (siRNA) to temporary knockdown early growth response protein 1 (Egr-1), hypoxia-inducible factor-1alpha (HIF-1alpha) and TF. The expression of CD2-associated protein (CD2AP) mRNA and phalloidin staining was examined to assess podocyte injury. RESULTS: Hypoxia increased mRNA expression of TF (6 h: 2.3 +/- 0.05 fold, p < 0.001, 24 h: 5.6 +/- 2.4 fold, p < 0.05) and suppressed TFPI (6 h: 0.54 +/- 0.04 fold, p < 0.05, 24 h: 0.24 +/- 0.06 fold, p < 0.001) compared with normoxia. Similarly, protein levels of TF were increased and TFPI were decreased. Egr-1 siRNA did not change TF mRNA expression. Pyrrolidine dithiocarbamate (PDTC), a nuclear factor kappa B (NF-kappaB) inhibitor, significantly reduced hypoxia induced TF expression, and HIF-1alpha knockdown further increased TF. Hypoxia resulted in decreased CD2AP and actin reorganization in podocytes, and these changes were attenuated by TF siRNA. CONCLUSION: Hypoxia increased the expression of TF in human podocytes NF-kappaB dependently. TF may have a critical role in the hypoxic podocyte injury. Twit Text FOAVip NF-kappaB-dependent increase in tissue factor expression is responsible for hypoxic podocyte injury ????Clin Exp Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=26715508 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26715508 #FOAvip English Wikipedia <ref>{{Cite pmid|26715508}}</ref> NF-kappaB-dependent increase in tissue factor expression is responsible for hypoxic podocyte injury #MMPMID26715508 Narita I; Shimada M; Yamabe H; Kinjo T; Tanno T; Nishizaki K; Kawai M; Nakamura M; Murakami R; Nakamura N; Tomita H; Saleem MA; Mathieson PW; Okumura K Clin Exp Nephrol 2016[Oct]; 20 (5): 679-688 PMID26715508show ga BACKGROUND: Fibrin deposition within glomeruli is commonly seen in kidney biopsy specimens, suggesting enhanced coagulant activity. Tissue factor (TF) is a coagulation factor which is also related to various biological effects, and TF is upregulated by hypoxia in cancer cells. Recently, hypoxic podocyte injury has been proposed, therefore, we investigated TF expression in hypoxia. METHODS: Conditionally immortalized human podocytes were differentiated and treated under hypoxic or normoxic conditions. mRNA expressions of TF and tissue factor pathway inhibitor (TFPI) were analyzed by quantitative RT-PCR. Protein levels of TF and TFPI were tested by enzyme-linked immunosorbent assay. We employed small interfering RNA (siRNA) to temporary knockdown early growth response protein 1 (Egr-1), hypoxia-inducible factor-1alpha (HIF-1alpha) and TF. The expression of CD2-associated protein (CD2AP) mRNA and phalloidin staining was examined to assess podocyte injury. RESULTS: Hypoxia increased mRNA expression of TF (6 h: 2.3 +/- 0.05 fold, p < 0.001, 24 h: 5.6 +/- 2.4 fold, p < 0.05) and suppressed TFPI (6 h: 0.54 +/- 0.04 fold, p < 0.05, 24 h: 0.24 +/- 0.06 fold, p < 0.001) compared with normoxia. Similarly, protein levels of TF were increased and TFPI were decreased. Egr-1 siRNA did not change TF mRNA expression. Pyrrolidine dithiocarbamate (PDTC), a nuclear factor kappa B (NF-kappaB) inhibitor, significantly reduced hypoxia induced TF expression, and HIF-1alpha knockdown further increased TF. Hypoxia resulted in decreased CD2AP and actin reorganization in podocytes, and these changes were attenuated by TF siRNA. CONCLUSION: Hypoxia increased the expression of TF in human podocytes NF-kappaB dependently. TF may have a critical role in the hypoxic podocyte injury. |Actin Cytoskeleton/metabolism[MESH] |Adaptor Proteins, Signal Transducing/genetics/metabolism[MESH] |Cell Hypoxia[MESH] |Cell Line[MESH] |Cobalt/pharmacology[MESH] |Cytoskeletal Proteins/genetics/metabolism[MESH] |Early Growth Response Protein 1/genetics/metabolism[MESH] |Fluorescent Antibody Technique[MESH] |Humans[MESH] |Hypoxia-Inducible Factor 1, alpha Subunit/genetics/metabolism[MESH] |Lipoproteins/genetics/metabolism[MESH] |NF-kappa B/antagonists & inhibitors/*metabolism[MESH] |Oxygen/*metabolism[MESH] |Phalloidine/metabolism[MESH] |Podocytes/drug effects/*metabolism/pathology[MESH] |Pyrrolidines/pharmacology[MESH] |RNA Interference[MESH] |RNA, Messenger/genetics/metabolism[MESH] |Signal Transduction[MESH] |Thiocarbamates/pharmacology[MESH] |Thromboplastin/genetics/*metabolism[MESH] |Time Factors[MESH] |Transfection[MESH]NF-kappaB-dependent increase in tissue factor expression is responsibl(epmc).pdf DeepDyve Pubget Overpricing