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10.1128/JVI.63.6.2422-2426.1989 http://scihub22266oqcxt.onion/10.1128/JVI.63.6.2422-2426.1989 2657097!250692!2657097     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol 1989 ; 63 (6): 2422-6 Nephropedia Template TP {{tp|p=2657097|t=1989. Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver |pdf=|usr=}}{{2657097}} gab.com Text Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=2657097 #FOAvip Erythroid lineage cells derived from fetal liver were demonstrated to be target cells for human parvovirus B19 infection. B19 virus antigen-positive serum was inoculated into primary cultures containing erythroid lineage cells enriched from fetal liver. The B19 virus antigen was detected on about 5% of cells in the culture by immunofluorescence staining, and the stained cells were identified as erythroid lineage cells by double staining with anti-B19 virus-positive serum and anti-erythroid lineage monoclonal antibody. The immunofluorescence staining study also revealed that the B19 virus antigen localized in the nucleus and the periphery of cytoplasm. We also detected B19 virus DNA, which was generated by replication in the infected cells, not only in the cells but also in the culture supernatants, in which the amount of B19 DNA increased depending on the period of culture, indicating that the cells infected with B19 virus produced B19 virus and released it into the medium. The ability of B19 virus released into the medium to infect fetal erythroid lineage cells was demonstrated quantitatively. Because of the absence of any cytopathic effect of B19 virus during culture periods of at least 15 days, this culture system should be useful in the study of B19 virus replication and in vitro generation of B19 virus. In addition, the present study may contribute to a better understanding of the pathogenesis of hydrops fetalis, which is probably associated with B19 virus infection during pregnancy. Twit Text FOAVip Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=2657097 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=2657097 #FOAvip English Wikipedia <ref>{{Cite pmid|2657097}}</ref> Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver #MMPMID2657097 Yaegashi N; Shiraishi H; Takeshita T; Nakamura M; Yajima A; Sugamura K J Virol 1989[Jun]; 63 (6): 2422-6 PMID2657097show ga Erythroid lineage cells derived from fetal liver were demonstrated to be target cells for human parvovirus B19 infection. B19 virus antigen-positive serum was inoculated into primary cultures containing erythroid lineage cells enriched from fetal liver. The B19 virus antigen was detected on about 5% of cells in the culture by immunofluorescence staining, and the stained cells were identified as erythroid lineage cells by double staining with anti-B19 virus-positive serum and anti-erythroid lineage monoclonal antibody. The immunofluorescence staining study also revealed that the B19 virus antigen localized in the nucleus and the periphery of cytoplasm. We also detected B19 virus DNA, which was generated by replication in the infected cells, not only in the cells but also in the culture supernatants, in which the amount of B19 DNA increased depending on the period of culture, indicating that the cells infected with B19 virus produced B19 virus and released it into the medium. The ability of B19 virus released into the medium to infect fetal erythroid lineage cells was demonstrated quantitatively. Because of the absence of any cytopathic effect of B19 virus during culture periods of at least 15 days, this culture system should be useful in the study of B19 virus replication and in vitro generation of B19 virus. In addition, the present study may contribute to a better understanding of the pathogenesis of hydrops fetalis, which is probably associated with B19 virus infection during pregnancy. |*DNA Replication[MESH] |*Virus Replication[MESH] |Cell Separation[MESH] |Cells, Cultured[MESH] |DNA, Viral/biosynthesis[MESH] |Erythrocytes[MESH] |Fluorescent Antibody Technique[MESH] |Hematopoietic Stem Cells/*microbiology[MESH] |Humans[MESH] |Liver/embryology[MESH] |Nucleic Acid Hybridization[MESH]Propagation of human parvovirus B19 in primary culture of erythroid li(epmc).pdf DeepDyve Pubget Overpricing