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suck abstract from ncbi


10.1002/9780471729259.mc15e02s37

http://scihub22266oqcxt.onion/10.1002/9780471729259.mc15e02s37
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26344219!4735735!26344219
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suck abstract from ncbi

pmid26344219      Curr+Protoc+Microbiol 2015 ; 37 (1): 15E.2.1-9
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  • Growth and Quantification of MERS-CoV Infection #MMPMID26344219
  • Coleman CM; Frieman MB
  • Curr Protoc Microbiol 2015[May]; 37 (1): 15E.2.1-9 PMID26344219show ga
  • Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging highly pathogenic respiratory virus. Although MERS-CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS-CoV and RNA products of replication in vitro. MERS-CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS-CoV are presented.
  • |Animals[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Middle East Respiratory Syndrome Coronavirus/genetics/*growth & development/*isolation & purification[MESH]
  • |RNA, Viral/analysis/isolation & purification[MESH]
  • |Vero Cells[MESH]
  • |Viral Load/*methods[MESH]


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