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10.1002/jmv.24366 http://scihub22266oqcxt.onion/10.1002/jmv.24366 26331371!7167125!26331371     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2016 ; 88 (4): 653-63 Nephropedia Template TP {{tp|p=26331371|t=2016. Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines |pdf=|usr=}}{{26331371}} gab.com Text Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=26331371 #FOAvip Hepatitis B virus (HBV) infection is one of the most serious and prevalent health problems worldwide. Current anti-HBV medications have a number of drawbacks, such as adverse effects and drug resistance; thus, novel potential anti-HBV reagents are needed. Selenium (Se) has been shown to be involved in both human immunodeficiency virus and hepatitis C virus infections, but its role in HBV infection remains unclear. To address this, sodium selenite (Na2SeO3 ) was applied to three HBV cell models: HepG2.2.15 cells, and HuH-7 cells transfected with either 1.1 or 1.3x HBV plasmids. Cytotoxicity of Na2SeO3 was examined by Cell Counting Kit-8. Levels of viral antigen expression, transcripts, and encapsidated viral DNA were measured by enzyme-linked immunosorbent assay, northern blot, and Southern blot, respectively. There was no obvious cytotoxicity in either HepG2.2.15 or HuH-7 cells with <2.5 microM Na2SeO3 . Below this concentration, Na2SeO3 suppressed HBsAg and HBeAg production, HBV transcript level, and amount of genomic DNA in all three tested models, and suppression level was enhanced in line with increases in Na2 SeO3 concentration or treatment time. Moreover, the inhibitory effect of Na2SeO3 on HBV replication can be further enhanced by combined treatment with lamivudine, entecavir, or adefovir. Thus, the present study clearly proves that Na2SeO3 suppresses HBV protein expression, transcription, and genome replication in hepatoma cell models in a dose- and time-dependent manner. Twit Text FOAVip Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=26331371 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26331371 #FOAvip English Wikipedia <ref>{{Cite pmid|26331371}}</ref> Sodium selenite suppresses hepatitis B virus transcription and replication in human hepatoma cell lines #MMPMID26331371 Cheng Z; Zhi X; Sun G; Guo W; Huang Y; Sun W; Tian X; Zhao F; Hu K J Med Virol 2016[Apr]; 88 (4): 653-63 PMID26331371show ga Hepatitis B virus (HBV) infection is one of the most serious and prevalent health problems worldwide. Current anti-HBV medications have a number of drawbacks, such as adverse effects and drug resistance; thus, novel potential anti-HBV reagents are needed. Selenium (Se) has been shown to be involved in both human immunodeficiency virus and hepatitis C virus infections, but its role in HBV infection remains unclear. To address this, sodium selenite (Na2SeO3 ) was applied to three HBV cell models: HepG2.2.15 cells, and HuH-7 cells transfected with either 1.1 or 1.3x HBV plasmids. Cytotoxicity of Na2SeO3 was examined by Cell Counting Kit-8. Levels of viral antigen expression, transcripts, and encapsidated viral DNA were measured by enzyme-linked immunosorbent assay, northern blot, and Southern blot, respectively. There was no obvious cytotoxicity in either HepG2.2.15 or HuH-7 cells with <2.5 microM Na2SeO3 . Below this concentration, Na2SeO3 suppressed HBsAg and HBeAg production, HBV transcript level, and amount of genomic DNA in all three tested models, and suppression level was enhanced in line with increases in Na2 SeO3 concentration or treatment time. Moreover, the inhibitory effect of Na2SeO3 on HBV replication can be further enhanced by combined treatment with lamivudine, entecavir, or adefovir. Thus, the present study clearly proves that Na2SeO3 suppresses HBV protein expression, transcription, and genome replication in hepatoma cell models in a dose- and time-dependent manner. |Antigens, Viral/analysis[MESH] |Antiviral Agents/*pharmacology/toxicity[MESH] |Blotting, Northern[MESH] |Blotting, Southern[MESH] |Cell Line, Tumor[MESH] |Cell Survival/drug effects[MESH] |DNA, Viral/analysis[MESH] |Drug Interactions[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Hepatitis B virus/*drug effects/genetics/*physiology[MESH] |Hepatocytes/*virology[MESH] |Humans[MESH] |RNA, Viral/analysis[MESH] |Sodium Selenite/*pharmacology/toxicity[MESH] |Transcription, Genetic/*drug effects[MESH]Sodium selenite suppresses hepatitis B virus transcription and replica(epmc).pdf DeepDyve Pubget Overpricing