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10.1080/15476286.2015.1085276 http://scihub22266oqcxt.onion/10.1080/15476286.2015.1085276 26325091!4829335!26325091     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RNA+Biol 2015 ; 12 (10): 1121-30 Nephropedia Template TP {{tp|p=26325091|t=2015. Induction of VEGFA mRNA translation by CoCl2 mediated by HuR |pdf=|usr=}}{{26325091}} gab.com Text Induction of VEGFA mRNA translation by CoCl2 mediated by HuR JO: RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=26325091 #FOAvip Vascular endothelial growth factor (VEGF) A is a master regulator of neovascularization and angiogenesis. VEGFA is potently induced by hypoxia and by pathological conditions including diabetic retinopathy and tumorigenesis. Fine-tuning of VEGFA expression by different stimuli is important for maintaining tissue vascularization and organ homeostasis. Here, we tested the effect of the hypoxia mimetic cobalt chloride (CoCl2) on VEGFA expression in human cervical carcinoma HeLa cells. We found that CoCl2 increased the levels of VEGFA mRNA and VEGFA protein without affecting VEGFA mRNA stability. Biotin pulldown analysis to capture the RNA-binding proteins (RBPs) bound to VEGFA mRNA followed by mass spectrometry analysis revealed that the RBP HuR [human antigen R, a member of the embryonic lethal abnormal vision (ELAV) family of proteins], interacts with VEGFA mRNA. VEGFA mRNA-tagging experiments showed that exposure to CoCl2 increases the interaction of HuR with VEGFA mRNA and promoted the colocalization of HuR and the distal part of the VEGFA 3'-untranslated region (UTR) in the cytoplasm. We propose that under hypoxia-like conditions, HuR enhances VEGFA mRNA translation. Twit Text FOAVip Induction of VEGFA mRNA translation by CoCl2 mediated by HuR ????RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=26325091 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26325091 #FOAvip English Wikipedia <ref>{{Cite pmid|26325091}}</ref> Induction of VEGFA mRNA translation by CoCl2 mediated by HuR #MMPMID26325091 Osera C; Martindale JL; Amadio M; Kim J; Yang X; Moad CA; Indig FE; Govoni S; Abdelmohsen K; Gorospe M; Pascale A RNA Biol 2015[]; 12 (10): 1121-30 PMID26325091show ga Vascular endothelial growth factor (VEGF) A is a master regulator of neovascularization and angiogenesis. VEGFA is potently induced by hypoxia and by pathological conditions including diabetic retinopathy and tumorigenesis. Fine-tuning of VEGFA expression by different stimuli is important for maintaining tissue vascularization and organ homeostasis. Here, we tested the effect of the hypoxia mimetic cobalt chloride (CoCl2) on VEGFA expression in human cervical carcinoma HeLa cells. We found that CoCl2 increased the levels of VEGFA mRNA and VEGFA protein without affecting VEGFA mRNA stability. Biotin pulldown analysis to capture the RNA-binding proteins (RBPs) bound to VEGFA mRNA followed by mass spectrometry analysis revealed that the RBP HuR [human antigen R, a member of the embryonic lethal abnormal vision (ELAV) family of proteins], interacts with VEGFA mRNA. VEGFA mRNA-tagging experiments showed that exposure to CoCl2 increases the interaction of HuR with VEGFA mRNA and promoted the colocalization of HuR and the distal part of the VEGFA 3'-untranslated region (UTR) in the cytoplasm. We propose that under hypoxia-like conditions, HuR enhances VEGFA mRNA translation. |*Protein Biosynthesis[MESH] |Cell Hypoxia/genetics[MESH] |Cobalt/pharmacology[MESH] |ELAV-Like Protein 1/biosynthesis/*genetics[MESH] |Gene Expression Regulation[MESH] |HeLa Cells[MESH] |Humans[MESH] |Mass Spectrometry[MESH] |Protein Processing, Post-Translational[MESH] |RNA Stability/genetics[MESH] |RNA, Messenger/genetics[MESH] |RNA-Binding Proteins/genetics[MESH] |Ribonucleoproteins/*genetics/metabolism[MESH]Induction of VEGFA mRNA translation by CoCl2 mediated by HuR (epmc).pdf DeepDyve Pubget Overpricing