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10.1002/jmv.24299

http://scihub22266oqcxt.onion/10.1002/jmv.24299
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26100490!7166673!26100490
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suck abstract from ncbi


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pmid26100490      J+Med+Virol 2016 ; 88 (1): 51-7
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  • Comparison of the conventional multiplex RT-PCR, real time RT-PCR and Luminex xTAG(R) RVP fast assay for the detection of respiratory viruses #MMPMID26100490
  • Choudhary ML; Anand SP; Tikhe SA; Walimbe AM; Potdar VA; Chadha MS; Mishra AC
  • J Med Virol 2016[Jan]; 88 (1): 51-7 PMID26100490show ga
  • Detection of respiratory viruses using polymerase chain reaction (PCR) is sensitive, specific and cost effective, having huge potential for patient management. In this study, the performance of an in-house developed conventional multiplex RT-PCR (mRT-PCR), real time RT-PCR (rtRT-PCR) and Luminex xTAG((R)) RVP fast assay (Luminex Diagnostics, Toronto, Canada) for the detection of respiratory viruses was compared. A total 310 respiratory clinical specimens predominantly from pediatric patients, referred for diagnosis of influenza A/H1N1pdm09 from August 2009 to March 2011 were tested to determine performance characteristic of the three methods. A total 193 (62.2%) samples were detected positive for one or more viruses by mRT-PCR, 175 (56.4%) samples by real time monoplex RT-PCR, and 138 (44.5%) samples by xTAG((R)) RVP fast assay. The overall sensitivity of mRT-PCR was 96.9% (95% CI: 93.5, 98.8), rtRT-PCR 87.9% (95% CI: 82.5, 92.1) and xTAG((R)) RVP fast was 68.3% (95% CI: 61.4, 74.6). Rhinovirus was detected most commonly followed by respiratory syncytial virus group B and influenza A/H1N1pdm09. The monoplex real time RT-PCR and in-house developed mRT-PCR are more sensitive, specific and cost effective than the xTAG((R)) RVP fast assay.
  • |Animals[MESH]
  • |Child, Preschool[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Infant[MESH]
  • |Male[MESH]
  • |Molecular Diagnostic Techniques/*methods[MESH]
  • |Multiplex Polymerase Chain Reaction/*methods[MESH]
  • |Real-Time Polymerase Chain Reaction/*methods[MESH]
  • |Respiratory Tract Infections/diagnosis/*virology[MESH]
  • |Retrospective Studies[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Virus Diseases/diagnosis/*virology[MESH]


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