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10.1002/jmv.24262 http://scihub22266oqcxt.onion/10.1002/jmv.24262 25959799!7166901!25959799     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2015 ; 87 (11): 1867-71 Nephropedia Template TP {{tp|p=25959799|t=2015. Evaluation of a PCR/ESI-MS platform to identify respiratory viruses from nasopharyngeal aspirates |pdf=|usr=}}{{25959799}} gab.com Text Evaluation of a PCR/ESI-MS platform to identify respiratory viruses from nasopharyngeal aspirates JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=25959799 #FOAvip Acute respiratory tract infection is a major cause of morbidity and mortality worldwide, particularly in infants and young children. High-throughput, accurate, broad-range tools for etiologic diagnosis are critical for effective epidemic control. In this study, the diagnostic capacities of an Ibis platform based on the PCR/ESI-MS assay were evaluated using clinical samples. Nasopharyngeal aspirates (NPAs) were collected from 120 children (<5 years old) who were hospitalized with lower respiratory tract infections between November 2010 and October 2011. The respiratory virus detection assay was performed using the PCR/ESI-MS assay and the DFA. The discordant PCR/ESI-MS and DFA results were resolved with RT-PCR plus sequencing. The overall agreement for PCR/ESI-MS and DFA was 98.3% (118/120). Compared with the results from DFA, the sensitivity and specificity of the PCR/ESI-MS assay were 100% and 97.5%, respectively. The PCR/ESI-MS assay also detected more multiple virus infections and revealed more detailed subtype information than DFA. Among the 12 original specimens with discordant results between PCR/ESI-MS and DFA, 11 had confirmed PCR/ESI-MS results. Thus, the PCR/ESI-MS assay is a high-throughput, sensitive, specific and promising method to detect and subtype conventional viruses in respiratory tract infections and allows rapid identification of mixed pathogens. Twit Text FOAVip Evaluation of a PCR/ESI-MS platform to identify respiratory viruses from nasopharyngeal aspirates ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=25959799 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25959799 #FOAvip English Wikipedia <ref>{{Cite pmid|25959799}}</ref> Evaluation of a PCR/ESI-MS platform to identify respiratory viruses from nasopharyngeal aspirates #MMPMID25959799 Lin Y; Fu Y; Xu M; Su L; Cao L; Xu J; Cheng X J Med Virol 2015[Nov]; 87 (11): 1867-71 PMID25959799show ga Acute respiratory tract infection is a major cause of morbidity and mortality worldwide, particularly in infants and young children. High-throughput, accurate, broad-range tools for etiologic diagnosis are critical for effective epidemic control. In this study, the diagnostic capacities of an Ibis platform based on the PCR/ESI-MS assay were evaluated using clinical samples. Nasopharyngeal aspirates (NPAs) were collected from 120 children (<5 years old) who were hospitalized with lower respiratory tract infections between November 2010 and October 2011. The respiratory virus detection assay was performed using the PCR/ESI-MS assay and the DFA. The discordant PCR/ESI-MS and DFA results were resolved with RT-PCR plus sequencing. The overall agreement for PCR/ESI-MS and DFA was 98.3% (118/120). Compared with the results from DFA, the sensitivity and specificity of the PCR/ESI-MS assay were 100% and 97.5%, respectively. The PCR/ESI-MS assay also detected more multiple virus infections and revealed more detailed subtype information than DFA. Among the 12 original specimens with discordant results between PCR/ESI-MS and DFA, 11 had confirmed PCR/ESI-MS results. Thus, the PCR/ESI-MS assay is a high-throughput, sensitive, specific and promising method to detect and subtype conventional viruses in respiratory tract infections and allows rapid identification of mixed pathogens. |Child, Preschool[MESH] |Clinical Laboratory Techniques/methods[MESH] |Female[MESH] |Fluorescent Antibody Technique, Direct[MESH] |Humans[MESH] |Infant[MESH] |Male[MESH] |Nasopharynx/*virology[MESH] |Polymerase Chain Reaction/*methods[MESH] |Respiratory Tract Infections/*diagnosis/virology[MESH] |Sensitivity and Specificity[MESH] |Spectrometry, Mass, Electrospray Ionization/*methods[MESH] |Virology/methods[MESH] |Virus Diseases/*diagnosis/virology[MESH]Evaluation of a PCR/ESI-MS platform to identify respiratory viruses fr(epmc).pdf DeepDyve Pubget Overpricing