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10.1208/s12248-015-9762-4 http://scihub22266oqcxt.onion/10.1208/s12248-015-9762-4 25924887DevelopmentandFit-for-PurposeValidationofaSolubleHumanProgrammedDeath-1ProteinAssay.!4476994!25924887; 1     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25924887; 1&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 400 Bad Request in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       AAPS+J;+Biochem+Med 2015 ; 17; 13 (4; 2): 976-87; 117-26 Nephropedia Template TP {{tp|p=25924887; 1|t=2015. Development and Fit-for-Purpose Validation of a Soluble Human Programmed Death-1 Protein Assay ; Formate assay in body fluids: application in methanol poisoning |pdf=|usr=}}{{25924887; 1}} gab.com Text Development and Fit-for-Purpose Validation of a Soluble Human Programmed Death-1 Protein Assay ; Formate assay in body fluids: application in methanol poisoning JO: AAPS J; Biochem Med http://www.kidney.de/pget.php?&tw=1&pmid=25924887; 1 #FOAvip Programmed death-1 (PD-1) protein is a co-inhibitory receptor which negatively regulates immune cell activation and permits tumors to evade normal immune defense. Anti-PD-1 antibodies have been shown to restore immune cell activation and effector function-an exciting breakthrough in cancer immunotherapy. Recent reports have documented a soluble form of PD-1 (sPD-1) in the circulation of normal and disease state individuals. A clinical assay to quantify sPD-1 would contribute to the understanding of sPD-1-function and facilitate the development of anti-PD-1 drugs. Here, we report the development and validation of a sPD-1 protein assay. The assay validation followed the framework for full validation of a biotherapeutic pharmacokinetic assay. A purified recombinant human PD-1 protein was characterized extensively and was identified as the assay reference material which mimics the endogenous analyte in structure and function. The lower limit of quantitation (LLOQ) was determined to be 100 pg/mL, with a dynamic range spanning three logs to 10,000 pg/mL. The intra- and inter-assay imprecision were </=15%, and the assay bias (percent deviation) was </=10%. Potential matrix effects were investigated in sera from both normal healthy volunteers and selected cancer patients. Bulk-prepared frozen standards and pre-coated Streptavidin plates were used in the assay to ensure consistency in assay performance over time. This assay appears to specifically measure total sPD-1 protein since the human anti-PD-1 antibody, nivolumab, and the endogenous ligands of PD-1 protein, PDL-1 and PDL-2, do not interfere with the assay. Twit Text FOAVip Development and Fit-for-Purpose Validation of a Soluble Human Programmed Death-1 Protein Assay ; Formate assay in body fluids: application in methanol poisoning ????AAPS J; Biochem Med http://www.kidney.de/pget.php?&tw=1&pmid=25924887; 1 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25924887; 1 #FOAvip English Wikipedia <ref>{{Cite pmid|25924887; 1}}</ref> Development and Fit-for-Purpose Validation of a Soluble Human Programmed Death-1 Protein Assay ; Formate assay in body fluids: application in methanol poisoning #MMPMID25924887; 1 Ni YG; Yuan X; Newitt JA; Peterson JE; Gleason CR; Haulenbeek J; Santockyte R; Lafont V; Marsilio F; Neely RJ; DeSilva B; Piccoli SP; Makar AB; McMartin KE; Palese M; Tephly TR AAPS J; Biochem Med 2015[Jul]; 17; 13 (4; 2): 976-87; 117-26 PMID25924887; 1show ga Programmed death-1 (PD-1) protein is a co-inhibitory receptor which negatively regulates immune cell activation and permits tumors to evade normal immune defense. Anti-PD-1 antibodies have been shown to restore immune cell activation and effector function-an exciting breakthrough in cancer immunotherapy. Recent reports have documented a soluble form of PD-1 (sPD-1) in the circulation of normal and disease state individuals. A clinical assay to quantify sPD-1 would contribute to the understanding of sPD-1-function and facilitate the development of anti-PD-1 drugs. Here, we report the development and validation of a sPD-1 protein assay. The assay validation followed the framework for full validation of a biotherapeutic pharmacokinetic assay. A purified recombinant human PD-1 protein was characterized extensively and was identified as the assay reference material which mimics the endogenous analyte in structure and function. The lower limit of quantitation (LLOQ) was determined to be 100 pg/mL, with a dynamic range spanning three logs to 10,000 pg/mL. The intra- and inter-assay imprecision were |Aldehyde Oxidoreductases/metabolism[MESH] |Animals[MESH] |Antibodies, Monoclonal/administration & dosage[MESH] |Biological Assay/*methods[MESH] |Body Fluids/*analysis[MESH] |Carbon Dioxide/blood[MESH] |Case-Control Studies[MESH] |Formates/blood/*poisoning[MESH] |HEK293 Cells[MESH] |Haplorhini[MESH] |Humans[MESH] |Hydrogen-Ion Concentration[MESH] |Kinetics[MESH] |Limit of Detection[MESH] |Methanol/blood[MESH] |Methods[MESH] |Neoplasms/blood[MESH] |Nivolumab[MESH] |Programmed Cell Death 1 Receptor/*analysis[MESH] |Pseudomonas/enzymology[MESH]Development and Fit-for-Purpose Validation of a Soluble Human Programm(epmc).pdf DeepDyve Pubget Overpricing