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10.1111/cmi.12446 http://scihub22266oqcxt.onion/10.1111/cmi.12446 25856684!7162271!25856684     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Microbiol 2015 ; 17 (9): 1391-404 Nephropedia Template TP {{tp|p=25856684|t=2015. Inhibition of viral protein translation by indomethacin in vesicular stomatitis virus infection: role of eIF2alpha kinase PKR |pdf=|usr=}}{{25856684}} gab.com Text Inhibition of viral protein translation by indomethacin in vesicular stomatitis virus infection: role of eIF2alpha kinase PKR JO: Cell Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=25856684 #FOAvip Indomethacin, a cyclooxygenase-1 and -2 inhibitor widely used in the clinic for its potent anti-inflammatory/analgesic properties, possesses antiviral activity against several viral pathogens; however, the mechanism of antiviral action remains elusive. We have recently shown that indomethacin activates the double-stranded RNA (dsRNA)-dependent protein kinase R (PKR) in human colon cancer cells. Because of the important role of PKR in the cellular defence response against viral infection, herein we investigated the effect of indomethacin on PKR activity during infection with the prototype rhabdovirus vesicular stomatitis virus. Indomethacin was found to activate PKR in an interferon- and dsRNA-independent manner, causing rapid (< 5 min) phosphorylation of eukaryotic initiation factor-2 alpha-subunit (eIF2alpha). These events resulted in shutting off viral protein translation and blocking viral replication (IC50 = 2 muM) while protecting host cells from virus-induced damage. Indomethacin did not affect eIF2alpha kinases PKR-like endoplasmic reticulum-resident protein kinase (PERK) and general control non-derepressible-2 (GCN2) kinase, and was unable to trigger eIF2alpha phosphorylation in the presence of PKR inhibitor 2-aminopurine. In addition, small-interfering RNA-mediated PKR gene silencing dampened the antiviral effect in indomethacin-treated cells. The results identify PKR as a critical target for the antiviral activity of indomethacin and indicate that eIF2alpha phosphorylation could be a key element in the broad spectrum antiviral activity of the drug. Twit Text FOAVip Inhibition of viral protein translation by indomethacin in vesicular stomatitis virus infection: role of eIF2alpha kinase PKR ????Cell Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=25856684 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25856684 #FOAvip English Wikipedia <ref>{{Cite pmid|25856684}}</ref> Inhibition of viral protein translation by indomethacin in vesicular stomatitis virus infection: role of eIF2alpha kinase PKR #MMPMID25856684 Amici C; La Frazia S; Brunelli C; Balsamo M; Angelini M; Santoro MG Cell Microbiol 2015[Sep]; 17 (9): 1391-404 PMID25856684show ga Indomethacin, a cyclooxygenase-1 and -2 inhibitor widely used in the clinic for its potent anti-inflammatory/analgesic properties, possesses antiviral activity against several viral pathogens; however, the mechanism of antiviral action remains elusive. We have recently shown that indomethacin activates the double-stranded RNA (dsRNA)-dependent protein kinase R (PKR) in human colon cancer cells. Because of the important role of PKR in the cellular defence response against viral infection, herein we investigated the effect of indomethacin on PKR activity during infection with the prototype rhabdovirus vesicular stomatitis virus. Indomethacin was found to activate PKR in an interferon- and dsRNA-independent manner, causing rapid (< 5 min) phosphorylation of eukaryotic initiation factor-2 alpha-subunit (eIF2alpha). These events resulted in shutting off viral protein translation and blocking viral replication (IC50 = 2 muM) while protecting host cells from virus-induced damage. Indomethacin did not affect eIF2alpha kinases PKR-like endoplasmic reticulum-resident protein kinase (PERK) and general control non-derepressible-2 (GCN2) kinase, and was unable to trigger eIF2alpha phosphorylation in the presence of PKR inhibitor 2-aminopurine. In addition, small-interfering RNA-mediated PKR gene silencing dampened the antiviral effect in indomethacin-treated cells. The results identify PKR as a critical target for the antiviral activity of indomethacin and indicate that eIF2alpha phosphorylation could be a key element in the broad spectrum antiviral activity of the drug. |Antiviral Agents/*metabolism[MESH] |Cell Line[MESH] |Enzyme Activators/metabolism[MESH] |Eukaryotic Initiation Factor-2/*metabolism[MESH] |Humans[MESH] |Indomethacin/*metabolism[MESH] |Inhibitory Concentration 50[MESH] |Phosphorylation[MESH] |Protein Biosynthesis/*drug effects[MESH] |Protein Processing, Post-Translational[MESH] |Vesiculovirus/*drug effects[MESH] |Viral Proteins/*biosynthesis[MESH]Inhibition of viral protein translation by indomethacin in vesicular s(epmc).pdf DeepDyve Pubget Overpricing