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10.1111/imm.12462

http://scihub22266oqcxt.onion/10.1111/imm.12462
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25762305!4515128!25762305
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suck abstract from ncbi


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pmid25762305      Immunology 2015 ; 145 (4): 476-84
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  • Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus #MMPMID25762305
  • Guo X; Deng Y; Chen H; Lan J; Wang W; Zou X; Hung T; Lu Z; Tan W
  • Immunology 2015[Aug]; 145 (4): 476-84 PMID25762305show ga
  • An ideal vaccine against mucosal pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV) should confer sustained, protective immunity at both systemic and mucosal levels. Here, we evaluated the in vivo systemic and mucosal antigen-specific immune responses induced by a single intramuscular or intragastric administration of recombinant adenoviral type 5 (Ad5) or type 41 (Ad41) -based vaccines expressing the MERS-CoV spike (S) protein. Intragastric administration of either Ad5-S or Ad41-S induced antigen-specific IgG and neutralizing antibody in serum; however, antigen-specific T-cell responses were not detected. In contrast, after a single intramuscular dose of Ad5-S or Ad41-S, functional antigen-specific T-cell responses were elicited in the spleen and pulmonary lymphocytes of the mice, which persisted for several months. Both rAd-based vaccines administered intramuscularly induced systemic humoral immune responses (neutralizing IgG antibodies). Our results show that a single dose of Ad5-S- or Ad41-S-based vaccines represents an appealing strategy for the control of MERS-CoV infection and transmission.
  • |*Immunity, Cellular[MESH]
  • |*Immunity, Mucosal[MESH]
  • |*Immunization[MESH]
  • |Adenoviridae/genetics/*immunology[MESH]
  • |Animals[MESH]
  • |Antibodies, Neutralizing/immunology[MESH]
  • |Antibodies, Viral/immunology[MESH]
  • |Base Sequence[MESH]
  • |Coronavirus Infections/genetics/immunology/*prevention & control/transmission[MESH]
  • |Humans[MESH]
  • |Immunoglobulin G/immunology[MESH]
  • |Mice[MESH]
  • |Middle East Respiratory Syndrome Coronavirus/genetics/*immunology[MESH]
  • |Molecular Sequence Data[MESH]
  • |Spike Glycoprotein, Coronavirus/genetics/*immunology[MESH]
  • |T-Lymphocytes/immunology[MESH]


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