Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1021/sb5003218 http://scihub22266oqcxt.onion/10.1021/sb5003218 25548949!4564286!25548949     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Synth+Biol 2015 ; 4 (6): 682-8 Nephropedia Template TP {{tp|p=25548949|t=2015. Expansion of bisindole biosynthetic pathways by combinatorial construction |pdf=|usr=}}{{25548949}} gab.com Text Expansion of bisindole biosynthetic pathways by combinatorial construction JO: ACS Synth Biol http://www.kidney.de/pget.php?&tw=1&pmid=25548949 #FOAvip Cladoniamides are indolotryptoline natural products that derive from indolocarbazole precursors. Here, we present a microbial platform to artificially redirect the cladoniamide pathway to generate unnatural bisindoles for drug discovery. Specifically, we target glycosyltransferase, halogenase, and oxidoreductase genes from the phylogenetically related indolocarbazole rebeccamycin and staurosporine pathways. We generate a series of novel compounds, reveal details about the substrate specificities of a number of enzymes, and set the stage for future efforts to develop new catalysts and compounds by engineering of bisindole genes. The strategy for structural diversification we use here could furthermore be applied to other natural product families with known biosynthetic genes. Twit Text FOAVip Expansion of bisindole biosynthetic pathways by combinatorial construction ????ACS Synth Biol http://www.kidney.de/pget.php?&tw=1&pmid=25548949 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25548949 #FOAvip English Wikipedia <ref>{{Cite pmid|25548949}}</ref> Expansion of bisindole biosynthetic pathways by combinatorial construction #MMPMID25548949 Du YL; Ryan KS ACS Synth Biol 2015[Jun]; 4 (6): 682-8 PMID25548949show ga Cladoniamides are indolotryptoline natural products that derive from indolocarbazole precursors. Here, we present a microbial platform to artificially redirect the cladoniamide pathway to generate unnatural bisindoles for drug discovery. Specifically, we target glycosyltransferase, halogenase, and oxidoreductase genes from the phylogenetically related indolocarbazole rebeccamycin and staurosporine pathways. We generate a series of novel compounds, reveal details about the substrate specificities of a number of enzymes, and set the stage for future efforts to develop new catalysts and compounds by engineering of bisindole genes. The strategy for structural diversification we use here could furthermore be applied to other natural product families with known biosynthetic genes. |Biological Products/chemistry/*metabolism[MESH] |Carbazoles/chemistry/metabolism[MESH] |Genetic Engineering[MESH] |Indole Alkaloids/chemistry/*metabolism[MESH] |Metabolic Engineering[MESH] |Multigene Family[MESH]Expansion of bisindole biosynthetic pathways by combinatorial construc(epmc).pdf DeepDyve Pubget Overpricing