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10.1152/ajprenal.00585.2014 http://scihub22266oqcxt.onion/10.1152/ajprenal.00585.2014 25520012!4360037!25520012     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25520012&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol+Renal+Physiol 2015 ; 308 (6): F572-8 Nephropedia Template TP {{tp|p=25520012|t=2015. Acute effects of aldosterone on the epithelial Na channel in rat kidney |pdf=|usr=}}{{25520012}} gab.com Text Acute effects of aldosterone on the epithelial Na channel in rat kidney JO: Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=25520012 #FOAvip The acute effects of aldosterone administration on epithelial Na channels (ENaC) in rat kidney were examined using electrophysiology and immunodetection. Animals received a single injection of aldosterone (20 mug/kg body wt), which reduced Na excretion over the next 3 h. Channel activity was assessed in principal cells of cortical collecting ducts as amiloride-sensitive whole cell clamp current (INa). INa averaged 100 pA/cell, 20-30% of that reported for the same preparation under conditions of chronic stimulation. INa was negligible in control animals that did not receive hormone. The acute physiological response correlated with changes in ENaC processing and trafficking. These effects included increases in the cleaved forms of alpha-ENaC and gamma-ENaC, assessed by Western blot, and increases in the surface expression of beta-ENaC and gamma-ENaC measured after surface protein biotinylation. These changes were qualitatively and quantitatively similar to those of chronic stimulation. This suggests that altered trafficking to or from the apical membrane is an early response to the hormone and that later increases in channel activity require stimulation of channels residing at the surface. Twit Text FOAVip Acute effects of aldosterone on the epithelial Na channel in rat kidney ????Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=25520012 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25520012 #FOAvip English Wikipedia <ref>{{Cite pmid|25520012}}</ref> Acute effects of aldosterone on the epithelial Na channel in rat kidney #MMPMID25520012 Frindt G; Palmer LG Am J Physiol Renal Physiol 2015[Mar]; 308 (6): F572-8 PMID25520012show ga The acute effects of aldosterone administration on epithelial Na channels (ENaC) in rat kidney were examined using electrophysiology and immunodetection. Animals received a single injection of aldosterone (20 mug/kg body wt), which reduced Na excretion over the next 3 h. Channel activity was assessed in principal cells of cortical collecting ducts as amiloride-sensitive whole cell clamp current (INa). INa averaged 100 pA/cell, 20-30% of that reported for the same preparation under conditions of chronic stimulation. INa was negligible in control animals that did not receive hormone. The acute physiological response correlated with changes in ENaC processing and trafficking. These effects included increases in the cleaved forms of alpha-ENaC and gamma-ENaC, assessed by Western blot, and increases in the surface expression of beta-ENaC and gamma-ENaC measured after surface protein biotinylation. These changes were qualitatively and quantitatively similar to those of chronic stimulation. This suggests that altered trafficking to or from the apical membrane is an early response to the hormone and that later increases in channel activity require stimulation of channels residing at the surface. |Aldosterone/*physiology[MESH] |Animals[MESH] |Biotinylation[MESH] |Epithelial Sodium Channels/*metabolism[MESH] |Female[MESH] |Kidney/*metabolism[MESH] |Patch-Clamp Techniques[MESH] |Potassium/urine[MESH] |Rats, Sprague-Dawley[MESH]Acute effects of aldosterone on the epithelial Na channel in rat kidne(epmc).pdf DeepDyve Pubget Overpricing