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10.1371/journal.pone.0111372 http://scihub22266oqcxt.onion/10.1371/journal.pone.0111372 25350118!4211740!25350118     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2014 ; 9 (10): e111372 Nephropedia Template TP {{tp|p=25350118|t=2014. Lack of negatively charged residues at the external mouth of Kir2 2 channels enable the voltage-dependent block by external Mg2+ |pdf=|usr=}}{{25350118}} gab.com Text Lack of negatively charged residues at the external mouth of Kir2 2 channels enable the voltage-dependent block by external Mg2+ JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25350118 #FOAvip Kir channels display voltage-dependent block by cytosolic cations such as Mg2+ and polyamines that causes inward rectification. In fact, cations can regulate K channel activity from both the extracellular and intracellular sides. Previous studies have provided insight into the up-regulation of Kir channel activity by extracellular K+ concentration. In contrast, extracellular Mg2+ has been found to reduce the amplitude of the single-channel current at milimolar concentrations. However, little is known about the molecular mechanism of Kir channel blockade by external Mg2+ and the relationship between the Mg2+ blockade and activity potentiation by permeant K+ ions. In this study, we applied an interactive approach between theory and experiment. Electrophysiological recordings on Kir2.2 and its mutants were performed by heterologous expression in Xenopus laevis oocytes. Our results confirmed that extracellular Mg2+ could reduce heterologously expressed WT Kir2.2 currents in a voltage dependent manner. The kinetics of inhibition and recovery of Mg2+ exhibit a 3 approximately 4s time constant. Molecular dynamics simulation results revealed a Mg2+ binding site located at the extracellular mouth of Kir2.2 that showed voltage-dependent Mg2+ binding. The mutants, G119D, Q126E and H128D, increased the number of permeant K+ ions and reduced the voltage-dependent blockade of Kir2.2 by extracellular Mg2+. Twit Text FOAVip Lack of negatively charged residues at the external mouth of Kir2 2 channels enable the voltage-dependent block by external Mg2+ ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=25350118 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25350118 #FOAvip English Wikipedia <ref>{{Cite pmid|25350118}}</ref> Lack of negatively charged residues at the external mouth of Kir2 2 channels enable the voltage-dependent block by external Mg2+ #MMPMID25350118 Li J; Xie X; Liu J; Yu H; Zhang S; Zhan Y; Zhang H; Logothetis DE; An H PLoS One 2014[]; 9 (10): e111372 PMID25350118show ga Kir channels display voltage-dependent block by cytosolic cations such as Mg2+ and polyamines that causes inward rectification. In fact, cations can regulate K channel activity from both the extracellular and intracellular sides. Previous studies have provided insight into the up-regulation of Kir channel activity by extracellular K+ concentration. In contrast, extracellular Mg2+ has been found to reduce the amplitude of the single-channel current at milimolar concentrations. However, little is known about the molecular mechanism of Kir channel blockade by external Mg2+ and the relationship between the Mg2+ blockade and activity potentiation by permeant K+ ions. In this study, we applied an interactive approach between theory and experiment. Electrophysiological recordings on Kir2.2 and its mutants were performed by heterologous expression in Xenopus laevis oocytes. Our results confirmed that extracellular Mg2+ could reduce heterologously expressed WT Kir2.2 currents in a voltage dependent manner. The kinetics of inhibition and recovery of Mg2+ exhibit a 3 approximately 4s time constant. Molecular dynamics simulation results revealed a Mg2+ binding site located at the extracellular mouth of Kir2.2 that showed voltage-dependent Mg2+ binding. The mutants, G119D, Q126E and H128D, increased the number of permeant K+ ions and reduced the voltage-dependent blockade of Kir2.2 by extracellular Mg2+. |*Gene Expression Regulation/drug effects[MESH] |Animals[MESH] |Binding Sites[MESH] |Chickens[MESH] |Crystallography, X-Ray[MESH] |Electrophysiological Phenomena[MESH] |Ion Channel Gating/drug effects[MESH] |Ions[MESH] |Magnesium/*chemistry[MESH] |Membrane Potentials/drug effects[MESH] |Molecular Dynamics Simulation[MESH] |Mutation[MESH] |Oocytes/*cytology[MESH] |Potassium Channels, Inwardly Rectifying/genetics/*physiology[MESH] |Potassium/chemistry[MESH]Lack of negatively charged residues at the external mouth of Kir2 2 ch(epmc).pdf DeepDyve Pubget Overpricing