Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText.
Kick-your-searchterm to multiple Engines kick-your-query now !>
A dictionary by aggregated review articles of nephrology, medicine and the life sciences
Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

suck abstract from ncbi


10.1038/aps.2014.68

http://scihub22266oqcxt.onion/10.1038/aps.2014.68
suck pdf from google scholar
25263338/?report=reader!4220072!25263338
unlimited free pdf from europmc25263338    free
PDF from PMC    free
html from PMC    free

suck abstract from ncbi


Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
pmid25263338      Acta+Pharmacol+Sin 2014 ; 35 (11): 1428-38
Nephropedia Template TP

gab.com Text

Twit Text FOAVip

Twit Text #

English Wikipedia


  • Fasudil regulates T cell responses through polarization of BV-2 cells in mice experimental autoimmune encephalomyelitis #MMPMID25263338
  • Chen C; Li YH; Zhang Q; Yu JZ; Zhao YF; Ma CG; Xiao BG
  • Acta Pharmacol Sin 2014[Nov]; 35 (11): 1428-38 PMID25263338show ga
  • AIM: Fasudil, a selective Rho kinase (ROCK) inhibitor, has been shown to alleviate the severity of experimental autoimmune encephalomyelitis (EAE) via attenuating demyelination and neuroinflammation. The aim of this study was to investigate the effects of fasudil on interactions between macrophages/microglia and T cells in a mice EAE model. METHODS: Mouse BV-2 microglia were treated with IFN-gamma and fasudil. Cell viability was detected with MTT assay. BV-2 microglia polarization was analyzed using flow cytometry. Cytokines and other proteins were detected with ELISA and Western blotting, respectively. Mice were immunized with MOG35-55 to induce EAE, and then treated with fasudil (40 mg/kg, ip) every other day from d 3 to d 27 pi. Encephalomyelitic T cells were prepared from the spleen of mice immunized with MOG35-55 on d 9 pi. RESULTS: Treatment of mouse BV-2 microglia with fasudil (15 mug/mL) induced significant phenotype polarization and functional plasticity, shifting M1 to M2 polarization. When co-cultured with the encephalomyelitic T cells, fasudil-treated BV-2 microglia significantly inhibited the proliferation of antigen-reactive T cells, and down-regulated IL-17-expressing CD4(+) T cells and IL-17 production. Furthermore, fasudil-treated BV-2 microglia significantly up-regulated CD4(+)CD25(high) and CD4(+)IL-10(+) regulatory T cells (Tregs) and IL-10 production, suggesting that the encephalomyelitic T cells had converted to Tregs. In EAE mice, fasudil administration significantly decreased both CD11b(+)iNOS(+) and CD11b(+)TNF-alpha(+) M1 microglia, and increased CD11b(+)IL-10(+) M2 microglia. CONCLUSION: Fasudil polarizes BV-2 microglia into M2 cells, which convert the encephalomyelitic T cells into Tregs in the mice EAE model.
  • |1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/*analogs & derivatives/pharmacology[MESH]
  • |Animals[MESH]
  • |Anti-Inflammatory Agents/*pharmacology[MESH]
  • |Cell Line[MESH]
  • |Cell Proliferation/drug effects[MESH]
  • |Cell Shape/drug effects[MESH]
  • |Cell Survival/drug effects[MESH]
  • |Coculture Techniques[MESH]
  • |Cytokines/metabolism[MESH]
  • |Dose-Response Relationship, Drug[MESH]
  • |Encephalomyelitis, Autoimmune, Experimental/chemically induced/*drug therapy/enzymology/immunology[MESH]
  • |Female[MESH]
  • |Inflammation Mediators/metabolism[MESH]
  • |Lymphocyte Activation/drug effects[MESH]
  • |Macrophages/drug effects/immunology[MESH]
  • |Mice, Inbred C57BL[MESH]
  • |Microglia/*drug effects/enzymology/immunology[MESH]
  • |Myelin-Oligodendrocyte Glycoprotein[MESH]
  • |Peptide Fragments[MESH]
  • |Phenotype[MESH]
  • |Protein Kinase Inhibitors/*pharmacology[MESH]
  • |T-Lymphocytes, Regulatory/*drug effects/immunology[MESH]


  • DeepDyve
  • Pubget Overpricing
  • suck abstract from ncbi

    Linkout box