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10.1016/0008-8749(89)90365-1 http://scihub22266oqcxt.onion/10.1016/0008-8749(89)90365-1 2521303!ä!2521303 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Immunol 1989 ; 118 (1): 157-65 Nephropedia Template TP {{tp|p=2521303|t=1989. Divalent metal requirement for soluble immune response suppressor (SIRS) activity |pdf=|usr=}}{{2521303}} gab.com Text Divalent metal requirement for soluble immune response suppressor (SIRS) activity JO: Cell Immunol http://www.kidney.de/pget.php?&tw=1&pmid=2521303 #FOAvip Soluble immune response suppressor (SIRS) is an immunosuppressive protein which requires activation to SIRSox by peroxide. This observation suggested that SIRS could be a metalloprotein. To investigate this possibility, purified hybridoma-derived SIRS was treated with chelating agents and dialyzed prior to activation with H2O2. EDTA, EGTA, or desferrioxamine prevented suppression of murine plaque-forming cell responses by SIRSox, whereas sodium citrate and penicillamine did not inhibit suppression. Suppressive activity was reconstituted by subsequent treatment of SIRS with FeSO4 (0.5 microM), NiSO4 (500 microM), or MgSO4 (500 microM), but not by FeCl3, MnSO4, CuSO4, ZnSO4, CaCl2, or CrCl3. Reconstitution of activity occurred only if FeSO4 was added at least 3 hr prior to treatment with H2O2. These data indicate that SIRS requires a divalent metal ion, probably ferrous iron, for activity, and suggest that SIRS is a metalloprotein. Twit Text FOAVip Divalent metal requirement for soluble immune response suppressor (SIRS) activity ????Cell Immunol http://www.kidney.de/pget.php?&tw=1&pmid=2521303 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=2521303 #FOAvip English Wikipedia <ref>{{Cite pmid|2521303}}</ref> Divalent metal requirement for soluble immune response suppressor (SIRS) activity #MMPMID2521303 Schnaper HW Cell Immunol 1989[Jan]; 118 (1): 157-65 PMID2521303show ga Soluble immune response suppressor (SIRS) is an immunosuppressive protein which requires activation to SIRSox by peroxide. This observation suggested that SIRS could be a metalloprotein. To investigate this possibility, purified hybridoma-derived SIRS was treated with chelating agents and dialyzed prior to activation with H2O2. EDTA, EGTA, or desferrioxamine prevented suppression of murine plaque-forming cell responses by SIRSox, whereas sodium citrate and penicillamine did not inhibit suppression. Suppressive activity was reconstituted by subsequent treatment of SIRS with FeSO4 (0.5 microM), NiSO4 (500 microM), or MgSO4 (500 microM), but not by FeCl3, MnSO4, CuSO4, ZnSO4, CaCl2, or CrCl3. Reconstitution of activity occurred only if FeSO4 was added at least 3 hr prior to treatment with H2O2. These data indicate that SIRS requires a divalent metal ion, probably ferrous iron, for activity, and suggest that SIRS is a metalloprotein. |Animals[MESH] |Cations, Divalent/*physiology[MESH] |Chelating Agents/pharmacology[MESH] |Hemolytic Plaque Technique[MESH] |Hydrogen Peroxide/pharmacology[MESH] |Metalloproteins/metabolism[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Oxidation-Reduction[MESH] |Suppressor Factors, Immunologic/*metabolism[MESH]Divalent metal requirement for soluble immune response suppressor (SIR(epmc).pdf DeepDyve Pubget Overpricing