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10.1002/cbic.201402250 http://scihub22266oqcxt.onion/10.1002/cbic.201402250 25209570!7162014!25209570     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Chembiochem 2014 ; 15 (15): 2268-74 Nephropedia Template TP {{tp|p=25209570|t=2014. Recombinase-based isothermal amplification of nucleic acids with self-avoiding molecular recognition systems (SAMRS) |pdf=|usr=}}{{25209570}} gab.com Text Recombinase-based isothermal amplification of nucleic acids with self-avoiding molecular recognition systems (SAMRS) JO: Chembiochem http://www.kidney.de/pget.php?&tw=1&pmid=25209570 #FOAvip Recombinase polymerase amplification (RPA) is an isothermal method to amplify nucleic acid sequences without the temperature cycling that classical PCR uses. Instead of using heat to denature the DNA duplex, RPA uses recombination enzymes to swap single-stranded primers into the duplex DNA product; these are then extended using a strand-displacing polymerase to complete the cycle. Because RPA runs at low temperatures, it never forces the system to recreate base-pairs following Watson-Crick rules, and therefore it produces undesired products that impede the amplification of the desired product, complicating downstream analysis. Herein, we show that most of these undesired side products can be avoided if the primers contain components of a self-avoiding molecular recognition system (SAMRS). Given the precision that is necessary in the recombination systems for them to function biologically, it is surprising that they accept SAMRS. SAMRS-RPA is expected to be a powerful tool within the range of amplification techniques available to scientists. Twit Text FOAVip Recombinase-based isothermal amplification of nucleic acids with self-avoiding molecular recognition systems (SAMRS) ????Chembiochem http://www.kidney.de/pget.php?&tw=1&pmid=25209570 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25209570 #FOAvip English Wikipedia <ref>{{Cite pmid|25209570}}</ref> Recombinase-based isothermal amplification of nucleic acids with self-avoiding molecular recognition systems (SAMRS) #MMPMID25209570 Sharma N; Hoshika S; Hutter D; Bradley KM; Benner SA Chembiochem 2014[Oct]; 15 (15): 2268-74 PMID25209570show ga Recombinase polymerase amplification (RPA) is an isothermal method to amplify nucleic acid sequences without the temperature cycling that classical PCR uses. Instead of using heat to denature the DNA duplex, RPA uses recombination enzymes to swap single-stranded primers into the duplex DNA product; these are then extended using a strand-displacing polymerase to complete the cycle. Because RPA runs at low temperatures, it never forces the system to recreate base-pairs following Watson-Crick rules, and therefore it produces undesired products that impede the amplification of the desired product, complicating downstream analysis. Herein, we show that most of these undesired side products can be avoided if the primers contain components of a self-avoiding molecular recognition system (SAMRS). Given the precision that is necessary in the recombination systems for them to function biologically, it is surprising that they accept SAMRS. SAMRS-RPA is expected to be a powerful tool within the range of amplification techniques available to scientists. |*Polymerase Chain Reaction[MESH] |*Temperature[MESH] |Nucleic Acids/*biosynthesis/*metabolism[MESH]Recombinase-based isothermal amplification of nucleic acids with self-(epmc).pdf DeepDyve Pubget Overpricing