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10.1111/1348-0421.12193 http://scihub22266oqcxt.onion/10.1111/1348-0421.12193 25154584!ä!25154584 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microbiol+Immunol 2014 ; 58 (11): 636-42 Nephropedia Template TP {{tp|p=25154584|t=2014. Heat stress is a potent stimulus for enhancing rescue efficiency of recombinant Borna disease virus |pdf=|usr=}}{{25154584}} gab.com Text Heat stress is a potent stimulus for enhancing rescue efficiency of recombinant Borna disease virus JO: Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=25154584 #FOAvip Recently developed vector systems based on Borna disease virus (BDV) hold promise as platforms for efficient and stable gene delivery to the central nervous system (CNS). However, because it currently takes several weeks to rescue recombinant BDV (rBDV), an improved rescue procedure would enhance the utility of this system. Heat stress reportedly enhances the rescue efficiency of other recombinant viruses. Here, heat stress was demonstrated to increase the amount of BDV genome in persistently BDV-infected cells without obvious cytotoxicity. Further analyses suggested that the effect of heat stress on BDV infection is not caused by an increase in the activity of BDV polymerase. More cells in which BDV replication occurs were obtained in the initial phase of rBDV rescue by using heat stress than when it was not used. Thus, heat stress is a useful improvement on the published rescue procedure for rBDV. The present findings may accelerate the practical use of BDV vector systems in basic science and the clinic and thus enable broader adoption of this viral vector, which is uniquely suited for gene delivery to the CNS. Twit Text FOAVip Heat stress is a potent stimulus for enhancing rescue efficiency of recombinant Borna disease virus ????Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=25154584 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25154584 #FOAvip English Wikipedia <ref>{{Cite pmid|25154584}}</ref> Heat stress is a potent stimulus for enhancing rescue efficiency of recombinant Borna disease virus #MMPMID25154584 Kojima S; Honda T; Matsumoto Y; Tomonaga K Microbiol Immunol 2014[Nov]; 58 (11): 636-42 PMID25154584show ga Recently developed vector systems based on Borna disease virus (BDV) hold promise as platforms for efficient and stable gene delivery to the central nervous system (CNS). However, because it currently takes several weeks to rescue recombinant BDV (rBDV), an improved rescue procedure would enhance the utility of this system. Heat stress reportedly enhances the rescue efficiency of other recombinant viruses. Here, heat stress was demonstrated to increase the amount of BDV genome in persistently BDV-infected cells without obvious cytotoxicity. Further analyses suggested that the effect of heat stress on BDV infection is not caused by an increase in the activity of BDV polymerase. More cells in which BDV replication occurs were obtained in the initial phase of rBDV rescue by using heat stress than when it was not used. Thus, heat stress is a useful improvement on the published rescue procedure for rBDV. The present findings may accelerate the practical use of BDV vector systems in basic science and the clinic and thus enable broader adoption of this viral vector, which is uniquely suited for gene delivery to the CNS. |*Genetic Vectors[MESH] |*Recombination, Genetic[MESH] |Animals[MESH] |Borna disease virus/genetics/*growth & development/isolation & purification/*radiation effects[MESH] |Cell Line[MESH] |Hot Temperature[MESH] |Humans[MESH] |Molecular Biology/*methods[MESH] |Reverse Genetics/*methods[MESH]Heat stress is a potent stimulus for enhancing rescue efficiency of re(epmc).pdf DeepDyve Pubget Overpricing