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10.1016/0166-0934(89)90148-1 http://scihub22266oqcxt.onion/10.1016/0166-0934(89)90148-1 2515204!?!2515204 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=2515204&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Virol+Methods 1989 ; 26 (2): 189-98 Nephropedia Template TP {{tp|p=2515204|t=1989. An amplified ELISA for the detection of parvovirus B19 IgM using monoclonal antibody to FITC |pdf=|usr=}}{{2515204}} gab.com Text An amplified ELISA for the detection of parvovirus B19 IgM using monoclonal antibody to FITC JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=2515204 #FOAvip A new M-antibody capture ELISA for the detection of specific IgM against parvovirus B19 is described. The test uses a monoclonal anti-fluorescein isothiocyanate (FITC) antibody conjugated to horseradish peroxidase to amplify the positive reactions. Serum samples (N = 823) submitted for B19 IgM assay were tested in parallel in the new ELISA and the standard B19 M-antibody capture radioimmunoassay (MACRIA) test. By both tests B19 IgM was detected in 38 (4.6%) samples, and not detected in 771 (94%) samples. One sample was positive in the ELISA test but negative in the RIA and of the 13 sera giving 'equivocal' results in the MACRIA, 6 were positive and 7 negative. If the RIA equivocal results were excluded, the ELISA showed 100% (38/38) sensitivity and 99.9% (771/772) specificity compared to the MACRIA test. The B19 IgM ELISA is a sensitive and specific test with better discrimination between anti B19 IgM positive and negative specimens than MACRIA. Twit Text FOAVip An amplified ELISA for the detection of parvovirus B19 IgM using monoclonal antibody to FITC ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=2515204 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=2515204 #FOAvip English Wikipedia <ref>{{Cite pmid|2515204}}</ref> An amplified ELISA for the detection of parvovirus B19 IgM using monoclonal antibody to FITC #MMPMID2515204 Brown KE; Buckley MM; Cohen BJ; Samuel D J Virol Methods 1989[Nov]; 26 (2): 189-98 PMID2515204show ga A new M-antibody capture ELISA for the detection of specific IgM against parvovirus B19 is described. The test uses a monoclonal anti-fluorescein isothiocyanate (FITC) antibody conjugated to horseradish peroxidase to amplify the positive reactions. Serum samples (N = 823) submitted for B19 IgM assay were tested in parallel in the new ELISA and the standard B19 M-antibody capture radioimmunoassay (MACRIA) test. By both tests B19 IgM was detected in 38 (4.6%) samples, and not detected in 771 (94%) samples. One sample was positive in the ELISA test but negative in the RIA and of the 13 sera giving 'equivocal' results in the MACRIA, 6 were positive and 7 negative. If the RIA equivocal results were excluded, the ELISA showed 100% (38/38) sensitivity and 99.9% (771/772) specificity compared to the MACRIA test. The B19 IgM ELISA is a sensitive and specific test with better discrimination between anti B19 IgM positive and negative specimens than MACRIA. |*Antibodies, Monoclonal[MESH] |*Enzyme-Linked Immunosorbent Assay[MESH] |Animals[MESH] |Fluorescein-5-isothiocyanate[MESH] |Fluoresceins[MESH] |Fluorescent Antibody Technique[MESH] |Humans[MESH] |Immunoglobulin M/*analysis[MESH] |Mice[MESH] |Parvoviridae/*immunology[MESH]An amplified ELISA for the detection of parvovirus B19 IgM using monoc(epmc).pdf DeepDyve Pubget Overpricing