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10.1111/tbed.12269

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suck abstract from ncbi


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pmid25098383      Transbound+Emerg+Dis 2014 ; 61 (5): 397-410
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  • Investigation into the role of potentially contaminated feed as a source of the first-detected outbreaks of porcine epidemic diarrhea in Canada #MMPMID25098383
  • Pasick J; Berhane Y; Ojkic D; Maxie G; Embury-Hyatt C; Swekla K; Handel K; Fairles J; Alexandersen S
  • Transbound Emerg Dis 2014[Oct]; 61 (5): 397-410 PMID25098383show ga
  • In January 2014, approximately 9 months following the initial detection of porcine epidemic diarrhea (PED) in the USA, the first case of PED was confirmed in a swine herd in south-western Ontario. A follow-up epidemiological investigation carried out on the initial and 10 subsequent Ontario PED cases pointed to feed as a common risk factor. As a result, several lots of feed and spray-dried porcine plasma (SDPP) used as a feed supplement were tested for the presence of PEDV genome by real-time RT-PCR assay. Several of these tested positive, supporting the notion that contaminated feed may have been responsible for the introduction of PEDV into Canada. These findings led us to conduct a bioassay experiment in which three PEDV-positive SDPP samples (from a single lot) and two PEDV-positive feed samples supplemented with this SDPP were used to orally inoculate 3-week-old piglets. Although the feed-inoculated piglets did not show any significant excretion of PEDV, the SDPP-inoculated piglets shed PEDV at a relatively high level for >/=9 days. Despite the fact that the tested PEDV genome positive feed did not result in obvious piglet infection in our bioassay experiment, contaminated feed cannot be ruled out as a likely source of this introduction in the field where many other variables may play a contributing role.
  • |*Porcine epidemic diarrhea virus[MESH]
  • |Animal Feed/*virology[MESH]
  • |Animals[MESH]
  • |Canada/epidemiology[MESH]
  • |Coronavirus Infections/*veterinary/virology[MESH]
  • |Diarrhea/epidemiology/*veterinary/virology[MESH]
  • |Disease Outbreaks/*veterinary[MESH]
  • |Food Contamination[MESH]
  • |Molecular Sequence Data[MESH]
  • |Swine[MESH]


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