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10.1007/s11655-014-1860-0 http://scihub22266oqcxt.onion/10.1007/s11655-014-1860-0 24972581!ä!24972581 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\24972581.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Chin+J+Integr+Med 2014 ; 20 (7): 540-5 Nephropedia Template TP {{tp|p=24972581|t=2014. Andrographolide as an anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway |pdf=|usr=}}{{24972581}} gab.com Text Andrographolide as an anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway JO: Chin J Integr Med http://www.kidney.de/pget.php?&tw=1&pmid=24972581 #FOAvip OBJECTIVE: To observe the anti-virus effects of andrographolide (AD) on the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) signaling pathway when immunological cells were infected with H1N1. METHODS: Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation, and immunological cells were harvested after cytokines stimulation. Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line (16HBE). The optimal concentration of AD was defined by methyl-thiazolyl-tetrazolium (MTT) assay. After the virus infected cell model was established, AD was added into the medium as a treatment intervention. After 24-h co-culture, cell supernatant was collected for interferon gamma (IFN-gamma) and interleukin-4 (IL-4) enzyme-linked immunosorbent assay (ELISA) detection while immunological cells for real-time polymerase chain reaction (RT-PCR). RESULTS: The optimal concentration of AD for anti-virus effect was 250 mug/mL. IL-4 and IFN-gamma in the supernatant and mRNA levels in RLRs pathway increased when cells was infected by virus, RIG-I, IFN-beta promoter stimulator-1 (IPS-1), interferon regulatory factor (IRF)-7, IRF-3 and nuclear transcription factor kappaB (NF-kappaB) mRNA levels increased significantly (P<0.05). When AD was added into co-culture medium, the levels of IL-4 and IFN-gamma were lower than those in the non-interference groups and the mRNA expression levels decreased, RIG-I, IPS-1, IRF-7, IRF-3 and NF-kappaB decreased significantly in each group with significant statistic differences (P<0.05). CONCLUSIONS: The RLRs mediated viral recognition provided a potential molecular target for acute viral infections and andrographolide could ameliorate H1N1 virus-induced cell mortality. And the antiviral effects might be related to its inhibition of viral-induced activation of the RLRs signaling pathway. Twit Text FOAVip Andrographolide as an anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway ????Chin J Integr Med http://www.kidney.de/pget.php?&tw=1&pmid=24972581 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24972581 #FOAvip English Wikipedia <ref>{{Cite pmid|24972581}}</ref> Andrographolide as an anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway #MMPMID24972581 Yu B; Dai CQ; Jiang ZY; Li EQ; Chen C; Wu XL; Chen J; Liu Q; Zhao CL; He JX; Ju DH; Chen XY Chin J Integr Med 2014[Jul]; 20 (7): 540-5 PMID24972581show ga OBJECTIVE: To observe the anti-virus effects of andrographolide (AD) on the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) signaling pathway when immunological cells were infected with H1N1. METHODS: Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation, and immunological cells were harvested after cytokines stimulation. Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line (16HBE). The optimal concentration of AD was defined by methyl-thiazolyl-tetrazolium (MTT) assay. After the virus infected cell model was established, AD was added into the medium as a treatment intervention. After 24-h co-culture, cell supernatant was collected for interferon gamma (IFN-gamma) and interleukin-4 (IL-4) enzyme-linked immunosorbent assay (ELISA) detection while immunological cells for real-time polymerase chain reaction (RT-PCR). RESULTS: The optimal concentration of AD for anti-virus effect was 250 mug/mL. IL-4 and IFN-gamma in the supernatant and mRNA levels in RLRs pathway increased when cells was infected by virus, RIG-I, IFN-beta promoter stimulator-1 (IPS-1), interferon regulatory factor (IRF)-7, IRF-3 and nuclear transcription factor kappaB (NF-kappaB) mRNA levels increased significantly (P<0.05). When AD was added into co-culture medium, the levels of IL-4 and IFN-gamma were lower than those in the non-interference groups and the mRNA expression levels decreased, RIG-I, IPS-1, IRF-7, IRF-3 and NF-kappaB decreased significantly in each group with significant statistic differences (P<0.05). CONCLUSIONS: The RLRs mediated viral recognition provided a potential molecular target for acute viral infections and andrographolide could ameliorate H1N1 virus-induced cell mortality. And the antiviral effects might be related to its inhibition of viral-induced activation of the RLRs signaling pathway. |Adaptor Proteins, Signal Transducing/genetics/metabolism[MESH] |Antiviral Agents/*pharmacology[MESH] |Cells, Cultured[MESH] |Coculture Techniques[MESH] |DEAD Box Protein 58[MESH] |DEAD-box RNA Helicases/genetics/*metabolism[MESH] |Dendritic Cells/drug effects/immunology/virology[MESH] |Diterpenes/*pharmacology[MESH] |Fetal Blood/cytology[MESH] |Humans[MESH] |Influenza A Virus, H1N1 Subtype/*drug effects/immunology[MESH] |Influenza, Human/*drug therapy/immunology/virology[MESH] |Interferon-beta/genetics/metabolism[MESH] |Interferon-gamma/metabolism[MESH] |Interleukin-4/metabolism[MESH] |Leukocytes, Mononuclear/*drug effects/immunology/virology[MESH] |Macrophages/drug effects/virology[MESH] |NF-kappa B/genetics/metabolism[MESH] |Promoter Regions, Genetic/drug effects/immunology[MESH] |RNA, Messenger/metabolism[MESH] |Receptors, Immunologic[MESH]Andrographolide as an anti-H1N1 drug and the mechanism related to reti(epmc).pdf DeepDyve Pubget Overpricing