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10.1002/jmv.23937 http://scihub22266oqcxt.onion/10.1002/jmv.23937 24797344!7166652!24797344     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2014 ; 86 (12): 2122-7 Nephropedia Template TP {{tp|p=24797344|t=2014. Comparison of sputum and nasopharyngeal swabs for detection of respiratory viruses |pdf=|usr=}}{{24797344}} gab.com Text Comparison of sputum and nasopharyngeal swabs for detection of respiratory viruses JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=24797344 #FOAvip Diagnostic tests for respiratory viral infections use traditionally either nasopharyngeal washes or swabs. Sputum is representative of the lower respiratory tract but is used rarely for viral testing. The aim of this study was to compare the detection rates of respiratory viruses from nasopharyngeal swabs and sputum using a multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR). Adults who were admitted or presented to the clinics of Gil Medical Center with acute respiratory symptoms were recruited from 1 November 2012 to 31 March 2013. Paired specimens of nasopharyngeal swabs and sputum were obtained from 154 subjects, and RNA was extracted and tested for 16 different respiratory viruses using the Anyplex II RV16 Detection kit (Seegene, Seoul, Korea). The positive rate was 53% (81/154) for nasopharyngeal swabs and 68% (105/154) for sputum (P < 0.001). One hundred thirty-four viruses were identified for 107 illnesses. Influenza A virus, RSV A, HRV, coronavirus OC43, and adenovirus were detected more frequently in sputum samples than in nasopharyngeal swabs (P < 0.001). Importantly, 12 of 44 (27%) influenza A infections and 11 of 27 (41%) RSV infections were positive in only sputum samples. The detection rates of respiratory viruses from sputum samples were significantly higher than those from nasopharyngeal swabs in adults using real-time multiplex RT-PCR. These findings suggest that sputum would benefit for the detection of respiratory viruses by nucleic acid amplification tests (NAATs) in patients who produce sputum. Further studies are needed to establish standardized RNA extraction methods from sputum samples. Twit Text FOAVip Comparison of sputum and nasopharyngeal swabs for detection of respiratory viruses ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=24797344 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24797344 #FOAvip English Wikipedia <ref>{{Cite pmid|24797344}}</ref> Comparison of sputum and nasopharyngeal swabs for detection of respiratory viruses #MMPMID24797344 Jeong JH; Kim KH; Jeong SH; Park JW; Lee SM; Seo YH J Med Virol 2014[Dec]; 86 (12): 2122-7 PMID24797344show ga Diagnostic tests for respiratory viral infections use traditionally either nasopharyngeal washes or swabs. Sputum is representative of the lower respiratory tract but is used rarely for viral testing. The aim of this study was to compare the detection rates of respiratory viruses from nasopharyngeal swabs and sputum using a multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR). Adults who were admitted or presented to the clinics of Gil Medical Center with acute respiratory symptoms were recruited from 1 November 2012 to 31 March 2013. Paired specimens of nasopharyngeal swabs and sputum were obtained from 154 subjects, and RNA was extracted and tested for 16 different respiratory viruses using the Anyplex II RV16 Detection kit (Seegene, Seoul, Korea). The positive rate was 53% (81/154) for nasopharyngeal swabs and 68% (105/154) for sputum (P < 0.001). One hundred thirty-four viruses were identified for 107 illnesses. Influenza A virus, RSV A, HRV, coronavirus OC43, and adenovirus were detected more frequently in sputum samples than in nasopharyngeal swabs (P < 0.001). Importantly, 12 of 44 (27%) influenza A infections and 11 of 27 (41%) RSV infections were positive in only sputum samples. The detection rates of respiratory viruses from sputum samples were significantly higher than those from nasopharyngeal swabs in adults using real-time multiplex RT-PCR. These findings suggest that sputum would benefit for the detection of respiratory viruses by nucleic acid amplification tests (NAATs) in patients who produce sputum. Further studies are needed to establish standardized RNA extraction methods from sputum samples. |Adult[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Multiplex Polymerase Chain Reaction/*methods[MESH] |Nasopharynx/*virology[MESH] |Respiratory Tract Infections/*diagnosis/*virology[MESH] |Reverse Transcriptase Polymerase Chain Reaction/methods[MESH] |Sensitivity and Specificity[MESH] |Sputum/*virology[MESH] |Virology/methods[MESH]Comparison of sputum and nasopharyngeal swabs for detection of respira(epmc).pdf DeepDyve Pubget Overpricing