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10.1002/mc.22131 http://scihub22266oqcxt.onion/10.1002/mc.22131 24436242!4101078!24436242     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Carcinog 2015 ; 54 (9): 698-706 Nephropedia Template TP {{tp|p=24436242|t=2015. Regulation of HIF-1 alpha by the proprotein convertases furin and PC7 in human squamous carcinoma cells |pdf=|usr=}}{{24436242}} gab.com Text Regulation of HIF-1 alpha by the proprotein convertases furin and PC7 in human squamous carcinoma cells JO: Mol Carcinog http://www.kidney.de/pget.php?&tw=1&pmid=24436242 #FOAvip Proprotein convertases (PC), a family of serine proteases, process cancer-related substrates such as growth factors, growth factor receptors, cell adhesion molecules, metalloproteinases, etc. HIF-1alpha is a major transcription factor involved in tumorigenesis by sensing intratumoral hypoxia. Furin (PCSK3) is one of the numerous target genes regulated by HIF-1alpha transactivation and its distribution into endosomal compartments and onto the cell surface can be triggered by hypoxia via HIF-1alpha. siRNAs to knockdown PCs were transfected into cells alone or in combination with different drug treatments. Protein and RNA expression levels were analyzed by Western blotting or RT-PCR, respectively. PC7 (PCSK7) and furin siRNAs upregulated HIF-1alpha protein under normoxic condition to a level similar to that obtained by cobalt chloride treatment, eventually leading to activation of VEGF-A synthesis in two human head and neck squamous cell carcinoma cell lines. The unchanged levels of HIF-1alpha mRNA expression under siRNA treatment and the additive HIF-1alpha induction of PC siRNAs and either cobalt chloride or the 26S ribosome inhibitor, MG-132, suggested a post-transcriptional PC-mediated regulation. Furthermore, cycloheximide chase showed that PC7/furin siRNA regulation occurred at the level of HIF-1alpha translation. A specific IGF-1R signaling inhibitor was able to attenuate the PC siRNA induction of HIF-1alpha, suggesting the involvement of the IGF-1R pathway. Thus, the data show that PCs regulate HIF-1alpha. Furin and PC7 siRNAs induced HIF-1alpha protein by increasing its translation, resulting in upregulation of VEGF-A. This finding may provide insight into intricate PC functions that seem to be independent from their substrate-processing activity. Twit Text FOAVip Regulation of HIF-1 alpha by the proprotein convertases furin and PC7 in human squamous carcinoma cells ????Mol Carcinog http://www.kidney.de/pget.php?&tw=1&pmid=24436242 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24436242 #FOAvip English Wikipedia <ref>{{Cite pmid|24436242}}</ref> Regulation of HIF-1 alpha by the proprotein convertases furin and PC7 in human squamous carcinoma cells #MMPMID24436242 Fu J; Zhang J; Gong Y; Testa CL; Klein-Szanto AJ Mol Carcinog 2015[Sep]; 54 (9): 698-706 PMID24436242show ga Proprotein convertases (PC), a family of serine proteases, process cancer-related substrates such as growth factors, growth factor receptors, cell adhesion molecules, metalloproteinases, etc. HIF-1alpha is a major transcription factor involved in tumorigenesis by sensing intratumoral hypoxia. Furin (PCSK3) is one of the numerous target genes regulated by HIF-1alpha transactivation and its distribution into endosomal compartments and onto the cell surface can be triggered by hypoxia via HIF-1alpha. siRNAs to knockdown PCs were transfected into cells alone or in combination with different drug treatments. Protein and RNA expression levels were analyzed by Western blotting or RT-PCR, respectively. PC7 (PCSK7) and furin siRNAs upregulated HIF-1alpha protein under normoxic condition to a level similar to that obtained by cobalt chloride treatment, eventually leading to activation of VEGF-A synthesis in two human head and neck squamous cell carcinoma cell lines. The unchanged levels of HIF-1alpha mRNA expression under siRNA treatment and the additive HIF-1alpha induction of PC siRNAs and either cobalt chloride or the 26S ribosome inhibitor, MG-132, suggested a post-transcriptional PC-mediated regulation. Furthermore, cycloheximide chase showed that PC7/furin siRNA regulation occurred at the level of HIF-1alpha translation. A specific IGF-1R signaling inhibitor was able to attenuate the PC siRNA induction of HIF-1alpha, suggesting the involvement of the IGF-1R pathway. Thus, the data show that PCs regulate HIF-1alpha. Furin and PC7 siRNAs induced HIF-1alpha protein by increasing its translation, resulting in upregulation of VEGF-A. This finding may provide insight into intricate PC functions that seem to be independent from their substrate-processing activity. |*Gene Expression Regulation, Neoplastic[MESH] |Carcinoma, Squamous Cell/*genetics/metabolism[MESH] |Cell Line, Tumor[MESH] |Down-Regulation[MESH] |Furin/*genetics/metabolism[MESH] |Head and Neck Neoplasms/*genetics/metabolism[MESH] |Humans[MESH] |Hypoxia-Inducible Factor 1, alpha Subunit/*genetics/metabolism[MESH] |Insulin-Like Growth Factor I/metabolism[MESH] |Protein Biosynthesis[MESH] |RNA Interference[MESH] |RNA, Small Interfering/genetics[MESH] |Signal Transduction[MESH] |Subtilisins/*genetics/metabolism[MESH] |Up-Regulation[MESH]Regulation of HIF-1 alpha by the proprotein convertases furin and PC7 (epmc).pdf DeepDyve Pubget Overpricing