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10.1016/j.bbrc.2014.01.005 http://scihub22266oqcxt.onion/10.1016/j.bbrc.2014.01.005 24434153!ä!24434153 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochem+Biophys+Res+Commun 2014 ; 444 (2): 121-7 Nephropedia Template TP {{tp|p=24434153|t=2014. Renoprotective effect of paricalcitol via a modulation of the TLR4-NF-kappaB pathway in ischemia/reperfusion-induced acute kidney injury |pdf=|usr=}}{{24434153}} gab.com Text Renoprotective effect of paricalcitol via a modulation of the TLR4-NF-kappaB pathway in ischemia/reperfusion-induced acute kidney injury JO: Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=24434153 #FOAvip BACKGROUND: The pathophysiology of ischemic acute kidney injury (AKI) is thought to include a complex interplay between vascular endothelial cell dysfunction, inflammation, and tubular cell damage. Several lines of evidence suggest a potential anti-inflammatory effect of vitamin D in various kidney injury models. In this study, we investigated the effect of paricalcitol, a synthetic vitamin D analog, on renal inflammation in a mouse model of ischemia/reperfusion (I/R) induced acute kidney injury (AKI). METHODS: Paricalcitol was administered via intraperitoneal (IP) injection at 24h before ischemia, and then I/R was performed through bilateral clamping of the renal pedicles. Twenty-four hours after I/R, mice were sacrificed for the evaluation of injury and inflammation. Additionally, an in vitro experiment using HK-2 cells was also performed to examine the direct effect of paricalcitol on tubular cells. RESULTS: Pre-treatment with paricalcitol attenuated functional deterioration and histological damage in I/R induced AKI, and significantly decreased tissue neutrophil and macrophage infiltration and the levels of chemokines, the pro-inflammatory cytokine interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1). It also decreased IR-induced upregulation of Toll-like receptor 4 (TLR4), and nuclear translocation of p65 subunit of NF-kappaB. Results from the in vitro study showed pre-treatment with paricalcitol suppressed the TNF-alpha-induced depletion of cytosolic IkappaB in HK-2 cells. CONCLUSION: These results demonstrate that pre-treatment with paricalcitol has a renoprotective effect in ischemic AKI, possibly by suppressing TLR4-NF-kappaB mediated inflammation. Twit Text FOAVip Renoprotective effect of paricalcitol via a modulation of the TLR4-NF-kappaB pathway in ischemia/reperfusion-induced acute kidney injury ????Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=24434153 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24434153 #FOAvip English Wikipedia <ref>{{Cite pmid|24434153}}</ref> Renoprotective effect of paricalcitol via a modulation of the TLR4-NF-kappaB pathway in ischemia/reperfusion-induced acute kidney injury #MMPMID24434153 Lee JW; Kim SC; Ko YS; Lee HY; Cho E; Kim MG; Jo SK; Cho WY; Kim HK Biochem Biophys Res Commun 2014[Feb]; 444 (2): 121-7 PMID24434153show ga BACKGROUND: The pathophysiology of ischemic acute kidney injury (AKI) is thought to include a complex interplay between vascular endothelial cell dysfunction, inflammation, and tubular cell damage. Several lines of evidence suggest a potential anti-inflammatory effect of vitamin D in various kidney injury models. In this study, we investigated the effect of paricalcitol, a synthetic vitamin D analog, on renal inflammation in a mouse model of ischemia/reperfusion (I/R) induced acute kidney injury (AKI). METHODS: Paricalcitol was administered via intraperitoneal (IP) injection at 24h before ischemia, and then I/R was performed through bilateral clamping of the renal pedicles. Twenty-four hours after I/R, mice were sacrificed for the evaluation of injury and inflammation. Additionally, an in vitro experiment using HK-2 cells was also performed to examine the direct effect of paricalcitol on tubular cells. RESULTS: Pre-treatment with paricalcitol attenuated functional deterioration and histological damage in I/R induced AKI, and significantly decreased tissue neutrophil and macrophage infiltration and the levels of chemokines, the pro-inflammatory cytokine interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1). It also decreased IR-induced upregulation of Toll-like receptor 4 (TLR4), and nuclear translocation of p65 subunit of NF-kappaB. Results from the in vitro study showed pre-treatment with paricalcitol suppressed the TNF-alpha-induced depletion of cytosolic IkappaB in HK-2 cells. CONCLUSION: These results demonstrate that pre-treatment with paricalcitol has a renoprotective effect in ischemic AKI, possibly by suppressing TLR4-NF-kappaB mediated inflammation. |Acute Kidney Injury/blood/etiology/*metabolism[MESH] |Animals[MESH] |Blotting, Western[MESH] |Cell Line[MESH] |Chemokine CCL2/metabolism[MESH] |Chemokines/metabolism[MESH] |Creatine/blood[MESH] |Ergocalciferols/*pharmacology[MESH] |Humans[MESH] |I-kappa B Kinase/metabolism[MESH] |Inflammation/blood/etiology/metabolism[MESH] |Interleukin-6/metabolism[MESH] |Kidney/drug effects/metabolism/pathology[MESH] |Macrophages/metabolism/pathology[MESH] |Male[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Neutrophils/metabolism/pathology[MESH] |Reperfusion Injury/complications[MESH] |Signal Transduction/*drug effects[MESH] |Toll-Like Receptor 4/*metabolism[MESH]Renoprotective effect of paricalcitol via a modulation of the TLR4-NF-(epmc).pdf DeepDyve Pubget Overpricing