Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Adv+Pharm+Bull 2013 ; 3 (1): 231-7 Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Extractive spectrophotometric determination of ambrisentan #MMPMID24312841
Kumar NS; Rani AP; Visalakshi T; Sekharan CB
Adv Pharm Bull 2013[]; 3 (1): 231-7 PMID24312841show ga
PURPOSE: Ambrisentan (ABS) is an antihypertensive drug used in the treatment of pulmonary atrial hypertension. The survey of literature for ABS revealed only two spectrophotometric methods for its quantification. The reported methods lack the sensitivity. This study is aimed at developing two sensitive extractive spectrophotometric methods for the determination of ABS in bulk and in tablets. METHODS: The proposed methods are based on the formation of colored chloroform extractable ion-pair complexes of ABS with methylene blue (MB method) and safranine O (SO method) in buffered solution at pH 9.8. The extracted complexes showed maximum absorbance at 525 and 515 nm for methylene blue and safranine O, respectively. RESULTS: In both the methods, the calibration curve was linear from 1-15 microg mL(-1) of drug. Apparent molar absorpitivities were 1.7911 x 10(5), 2.3272 x 10(5) L mol(-1) cm(-1); Sandell's sensitivities were 0.0215, 0.0162 microg cm(-2); LOD were 0.182, 0.175 microg mL(-1); LOQ were 0.551, 0.531 microg mL(-1) for methods MB and SO, respectively. The relative standard deviation and percent recovery ranged from 0.206-1.310% and 99.0-101.5%, respectively. CONCLUSION: The results demonstrate that the proposed methods are sensitive, precise, accurate and inexpensive. These methods can easily be used for the assay of ABS in quality control laboratories.
free
free
free
Adv+Pharm+Bull 2013 ; 3 (1): 231-7
