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10.1007/s00345-013-1208-y http://scihub22266oqcxt.onion/10.1007/s00345-013-1208-y 24258314!ä!24258314 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       World+J+Urol 2014 ; 32 (5): 1303-11 Nephropedia Template TP {{tp|p=24258314|t=2014. Suppression of renal TRPM7 may alleviate kidney injury in the renal transplantation |pdf=|usr=}}{{24258314}} gab.com Text Suppression of renal TRPM7 may alleviate kidney injury in the renal transplantation JO: World J Urol http://www.kidney.de/pget.php?&tw=1&pmid=24258314 #FOAvip PURPOSE: The aim of this study was to investigate the effect of renal cortex transient receptor potential melastatin 7 (TRPM7) suppression on renal ischemia reperfusion injury induced by transplantation in mice. METHODS: M7shRNA was used to decrease the expression of TRPM7 in NRK-52e cells. The mice were subjected to renal intra-parenchymal injection with lentivirus containing M7shRNA to produce hypo-expression of TRPM7 in renal cortex. Cell hypoxia mode and syngeneic renal transplantation in vivo were established. Then the effects of M7shRNA were measured by fluorescent probe for reactive oxygen species (ROS), intracellular calcium and magnesium; Western blot was applied for p38-MAPKs and Bax expression in cell studies. In vivo studies, mice were killed 24 h, 48 h, 72 h, 7 days and 21 days, respectively, after transplantation and the kidneys were dissected. Serum creatinine was measured, and the H&E, Masson's trichrome staining, TUNEL, Kim-1 and alpha-smooth muscle actin were used to evaluate pathological change. RESULTS: In cell model of hypoxia, the level of ROS in NRK-52e-M7shRNA was significantly lower than that in both NRK-52e and NRK-52e control cells, while the activation of p38-MAPKs was limited. In renal transplanted mice, renal function of M7shRNA group was conspicuously better than PBS- and vector-control-treated group. The histological examination showed that renal tubule injury and interstitial fibrosis were lower in M7shRNA-treated group compared with PBS and vector-control group. CONCLUSIONS: Suppression of renal cortex TRPM7 could alleviate kidney injury induced by transplantation in mice. The mechanism may involve reducing the early stage of ischemia reperfusion injury by inhibition of intracellular Ca(2+), Mg(2+) and ROS. Twit Text FOAVip Suppression of renal TRPM7 may alleviate kidney injury in the renal transplantation ????World J Urol http://www.kidney.de/pget.php?&tw=1&pmid=24258314 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24258314 #FOAvip English Wikipedia <ref>{{Cite pmid|24258314}}</ref> Suppression of renal TRPM7 may alleviate kidney injury in the renal transplantation #MMPMID24258314 Meng Z; Cao R; Wang Y; Cao H; Liu T; Yang Z; Wang X World J Urol 2014[Oct]; 32 (5): 1303-11 PMID24258314show ga PURPOSE: The aim of this study was to investigate the effect of renal cortex transient receptor potential melastatin 7 (TRPM7) suppression on renal ischemia reperfusion injury induced by transplantation in mice. METHODS: M7shRNA was used to decrease the expression of TRPM7 in NRK-52e cells. The mice were subjected to renal intra-parenchymal injection with lentivirus containing M7shRNA to produce hypo-expression of TRPM7 in renal cortex. Cell hypoxia mode and syngeneic renal transplantation in vivo were established. Then the effects of M7shRNA were measured by fluorescent probe for reactive oxygen species (ROS), intracellular calcium and magnesium; Western blot was applied for p38-MAPKs and Bax expression in cell studies. In vivo studies, mice were killed 24 h, 48 h, 72 h, 7 days and 21 days, respectively, after transplantation and the kidneys were dissected. Serum creatinine was measured, and the H&E, Masson's trichrome staining, TUNEL, Kim-1 and alpha-smooth muscle actin were used to evaluate pathological change. RESULTS: In cell model of hypoxia, the level of ROS in NRK-52e-M7shRNA was significantly lower than that in both NRK-52e and NRK-52e control cells, while the activation of p38-MAPKs was limited. In renal transplanted mice, renal function of M7shRNA group was conspicuously better than PBS- and vector-control-treated group. The histological examination showed that renal tubule injury and interstitial fibrosis were lower in M7shRNA-treated group compared with PBS and vector-control group. CONCLUSIONS: Suppression of renal cortex TRPM7 could alleviate kidney injury induced by transplantation in mice. The mechanism may involve reducing the early stage of ischemia reperfusion injury by inhibition of intracellular Ca(2+), Mg(2+) and ROS. |*Kidney Transplantation[MESH] |Animals[MESH] |Cells, Cultured[MESH] |Kidney/*blood supply/metabolism[MESH] |Male[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Postoperative Complications/*prevention & control[MESH] |Reperfusion Injury/*prevention & control[MESH]Suppression of renal TRPM7 may alleviate kidney injury in the renal t(epmc).pdf DeepDyve Pubget Overpricing