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10.1371/journal.pone.0076605 http://scihub22266oqcxt.onion/10.1371/journal.pone.0076605 24098539!3789701!24098539     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2013 ; 8 (10): e76605 Nephropedia Template TP {{tp|p=24098539|t=2013. Autophagy induced by HIF1alpha overexpression supports trophoblast invasion by supplying cellular energy |pdf=|usr=}}{{24098539}} gab.com Text Autophagy induced by HIF1alpha overexpression supports trophoblast invasion by supplying cellular energy JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=24098539 #FOAvip Extravillous trophoblasts (EVTs) characterize the invasion of the maternal decidua under low oxygen and poor nutrition at the early feto-maternal interface to establish a successful pregnancy. We previously reported that autophagy in EVTs was activated under 2% O2 in vitro, and autophagy activation was also observed in EVTs at the early feto-maternal interface in vivo. Here, we show that autophagy is an energy source for the invasion of EVTs. Cobalt chloride (CoCl2), which induces hypoxia inducible factor 1alpha (HIF1alpha) overexpression, activated autophagy in HTR8/SVneo cells, an EVT cell line. The number of invading HTR8-ATG4B(C74A) cells, an autophagy-deficient EVT cell line, was markedly reduced by 81 percent with the CoCl2 treatment through the suppression of MMP9 level, although CoCl2 did not affect the cellular invasion of HTR8-mStrawberry cells, a control cell line. HTR8-ATG4B(C74A) cells treated with CoCl2 showed a decrease in cellular adenosine triphosphate (ATP) levels and a compensatory increase in the expression of purinergic receptor P2X ligand-gated ion channel 7 (P2RX7), which is stimulated with ATP, whereas HTR8-mStrawberry cells maintained cellular ATP levels and did not affect P2RX7 expression. Furthermore, the decreased invasiveness of HTR8-ATG4B(C74A) cells treated with CoCl2 was neutralized by ATP supplementation to the level of HTR8-ATG4B(C74A) cells treated without CoCl2. These results suggest that autophagy plays a role in maintaining homeostasis by countervailing HIF1alpha-mediated cellular energy consumption in EVTs. Twit Text FOAVip Autophagy induced by HIF1alpha overexpression supports trophoblast invasion by supplying cellular energy ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=24098539 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24098539 #FOAvip English Wikipedia <ref>{{Cite pmid|24098539}}</ref> Autophagy induced by HIF1alpha overexpression supports trophoblast invasion by supplying cellular energy #MMPMID24098539 Yamanaka-Tatematsu M; Nakashima A; Fujita N; Shima T; Yoshimori T; Saito S PLoS One 2013[]; 8 (10): e76605 PMID24098539show ga Extravillous trophoblasts (EVTs) characterize the invasion of the maternal decidua under low oxygen and poor nutrition at the early feto-maternal interface to establish a successful pregnancy. We previously reported that autophagy in EVTs was activated under 2% O2 in vitro, and autophagy activation was also observed in EVTs at the early feto-maternal interface in vivo. Here, we show that autophagy is an energy source for the invasion of EVTs. Cobalt chloride (CoCl2), which induces hypoxia inducible factor 1alpha (HIF1alpha) overexpression, activated autophagy in HTR8/SVneo cells, an EVT cell line. The number of invading HTR8-ATG4B(C74A) cells, an autophagy-deficient EVT cell line, was markedly reduced by 81 percent with the CoCl2 treatment through the suppression of MMP9 level, although CoCl2 did not affect the cellular invasion of HTR8-mStrawberry cells, a control cell line. HTR8-ATG4B(C74A) cells treated with CoCl2 showed a decrease in cellular adenosine triphosphate (ATP) levels and a compensatory increase in the expression of purinergic receptor P2X ligand-gated ion channel 7 (P2RX7), which is stimulated with ATP, whereas HTR8-mStrawberry cells maintained cellular ATP levels and did not affect P2RX7 expression. Furthermore, the decreased invasiveness of HTR8-ATG4B(C74A) cells treated with CoCl2 was neutralized by ATP supplementation to the level of HTR8-ATG4B(C74A) cells treated without CoCl2. These results suggest that autophagy plays a role in maintaining homeostasis by countervailing HIF1alpha-mediated cellular energy consumption in EVTs. |Adenosine Triphosphate/*metabolism[MESH] |Autophagy/*genetics[MESH] |Cell Adhesion[MESH] |Cell Line[MESH] |Cell Movement[MESH] |Cobalt/pharmacology[MESH] |Decidua/cytology/metabolism[MESH] |Energy Metabolism[MESH] |Female[MESH] |Gene Expression Regulation, Developmental[MESH] |Humans[MESH] |Hypoxia-Inducible Factor 1, alpha Subunit/agonists/*genetics/metabolism[MESH] |Hypoxia/metabolism[MESH] |Matrix Metalloproteinase 9/genetics/metabolism[MESH] |Pregnancy[MESH] |Pregnancy Trimester, First[MESH] |Receptors, Purinergic P2X7/genetics/metabolism[MESH] |Signal Transduction[MESH]Autophagy induced by HIF1alpha overexpression supports trophoblast inv(epmc).pdf DeepDyve Pubget Overpricing