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10.1152/ajpcell.00273.2013 http://scihub22266oqcxt.onion/10.1152/ajpcell.00273.2013 24025865!3840200!24025865     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=24025865&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Am+J+Physiol+Cell+Physiol 2013 ; 305 (10): C999-C1008 Nephropedia Template TP {{tp|p=24025865|t=2013. TRP channel Ca(2+) sparklets: fundamental signals underlying endothelium-dependent hyperpolarization |pdf=|usr=}}{{24025865}} gab.com Text TRP channel Ca(2+) sparklets: fundamental signals underlying endothelium-dependent hyperpolarization JO: Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=24025865 #FOAvip Important functions of the vascular endothelium, including permeability, production of antithrombotic factors, and control of vascular tone, are regulated by changes in intracellular Ca(2+). The molecular identities and regulation of Ca(2+) influx channels in the endothelium are incompletely understood, in part because of experimental difficulties associated with application of patch-clamp electrophysiology to native endothelial cells. However, advances in confocal and total internal reflection fluorescence microscopy and the development of fast, high-affinity Ca(2+)-binding fluorophores have recently allowed for direct visualization and characterization of single-channel transient receptor potential (TRP) channel Ca(2+) influx events in endothelial cells. These events, called "TRP channel Ca(2+) sparklets," have been optically recorded from primary endothelial cells and the intact endothelium, and the biophysical properties and fundamental significance of these Ca(2+) signals in vasomotor regulation have been characterized. This review will first briefly discuss the role of endothelial cell TRP channel Ca(2+) influx in endothelium-dependent vasodilation, describe improved methods for recording unitary TRP channel activity using optical methods, and highlight discoveries regarding the regulation and physiological significance of TRPV4 Ca(2+) sparklets in the vascular endothelium enabled by this new technology. Perspectives on the potential use of these techniques to evaluate changes in TRP channel Ca(2+) influx activity associated with endothelial dysfunction are offered. Twit Text FOAVip TRP channel Ca(2+) sparklets: fundamental signals underlying endothelium-dependent hyperpolarization ????Am J Physiol Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=24025865 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24025865 #FOAvip English Wikipedia <ref>{{Cite pmid|24025865}}</ref> TRP channel Ca(2+) sparklets: fundamental signals underlying endothelium-dependent hyperpolarization #MMPMID24025865 Sullivan MN; Earley S Am J Physiol Cell Physiol 2013[Nov]; 305 (10): C999-C1008 PMID24025865show ga Important functions of the vascular endothelium, including permeability, production of antithrombotic factors, and control of vascular tone, are regulated by changes in intracellular Ca(2+). The molecular identities and regulation of Ca(2+) influx channels in the endothelium are incompletely understood, in part because of experimental difficulties associated with application of patch-clamp electrophysiology to native endothelial cells. However, advances in confocal and total internal reflection fluorescence microscopy and the development of fast, high-affinity Ca(2+)-binding fluorophores have recently allowed for direct visualization and characterization of single-channel transient receptor potential (TRP) channel Ca(2+) influx events in endothelial cells. These events, called "TRP channel Ca(2+) sparklets," have been optically recorded from primary endothelial cells and the intact endothelium, and the biophysical properties and fundamental significance of these Ca(2+) signals in vasomotor regulation have been characterized. This review will first briefly discuss the role of endothelial cell TRP channel Ca(2+) influx in endothelium-dependent vasodilation, describe improved methods for recording unitary TRP channel activity using optical methods, and highlight discoveries regarding the regulation and physiological significance of TRPV4 Ca(2+) sparklets in the vascular endothelium enabled by this new technology. Perspectives on the potential use of these techniques to evaluate changes in TRP channel Ca(2+) influx activity associated with endothelial dysfunction are offered. |Animals[MESH] |Calcium/metabolism[MESH] |Endothelium/*physiology[MESH] |Membrane Potentials[MESH]TRP channel Ca(2+) sparklets: fundamental signals underlying endothel(epmc).pdf DeepDyve Pubget Overpricing