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10.1002/jmv.23648

http://scihub22266oqcxt.onion/10.1002/jmv.23648
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23861138!7167105!23861138
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suck abstract from ncbi


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pmid23861138      J+Med+Virol 2013 ; 85 (10): 1852-9
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  • Concurrent detection of other respiratory viruses in children shedding viable human respiratory syncytial virus #MMPMID23861138
  • Gagliardi TB; Paula FE; Iwamoto MA; Proenca-Modena JL; Santos AE; Camara AA; Cervi MC; Cintra OA; Arruda E
  • J Med Virol 2013[Oct]; 85 (10): 1852-9 PMID23861138show ga
  • Human respiratory syncytial virus (HRSV) is an important cause of respiratory disease. The majority of studies addressing the importance of virus co-infections to the HRSV-disease have been based on the detection of HRSV by RT-PCR, which may not distinguish current replication from prolonged shedding of remnant RNA from previous HRSV infections. To assess whether co-detections of other common respiratory viruses are associated with increased severity of HRSV illnesses from patients who were shedding viable-HRSV, nasopharyngeal aspirates from children younger than 5 years who sought medical care for respiratory infections in Ribeirao Preto (Brazil) were tested for HRSV by immunofluorescence, RT-PCR and virus isolation in cell culture. All samples with viable-HRSV were tested further by PCR for other respiratory viruses. HRSV-disease severity was assessed by a clinical score scale. A total of 266 samples from 247 children were collected and 111 (42%) were HRSV-positive. HRSV was isolated from 70 (63%), and 52 (74%) of them were positive for at least one additional virus. HRSV-positive diseases were more severe than HRSV-negative ones, but there was no difference in disease severity between patients with viable-HRSV and those HRSV-positives by RT-PCR. Co-detection of other viruses did not correlate with increased disease severity. HRSV isolation in cell culture does not seem to be superior to RT-PCR to distinguish infections associated with HRSV replication in studies of clinical impact of HRSV. A high rate of co-detection of other respiratory viruses was found in samples with viable-HRSV, but this was not associated with more severe HRSV infection.
  • |Brazil[MESH]
  • |Child, Preschool[MESH]
  • |Coinfection/pathology/*virology[MESH]
  • |Female[MESH]
  • |Fluorescent Antibody Technique[MESH]
  • |Humans[MESH]
  • |Infant[MESH]
  • |Infant, Newborn[MESH]
  • |Male[MESH]
  • |Nasopharynx/virology[MESH]
  • |RNA Viruses/*isolation & purification[MESH]
  • |Respiratory Tract Infections/pathology/*virology[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |Severity of Illness Index[MESH]
  • |Virus Cultivation[MESH]


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