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10.1021/sb300050j http://scihub22266oqcxt.onion/10.1021/sb300050j 23656278!3653836!23656278     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Synth+Biol 2012 ; 1 (12): 569-75 Nephropedia Template TP {{tp|p=23656278|t=2012. Dimerization-dependent green and yellow fluorescent proteins |pdf=|usr=}}{{23656278}} gab.com Text Dimerization-dependent green and yellow fluorescent proteins JO: ACS Synth Biol http://www.kidney.de/pget.php?&tw=1&pmid=23656278 #FOAvip Dimerization-dependent fluorescent proteins (ddFP) are a recently introduced class of genetically encoded reporters that can be used for the detection of protein interactions in live cells. The progenitor of this class of tools was a red fluorescent ddFP (ddRFP) derived from a homodimeric variant of Discosoma red fluorescent protein. Here, we describe the engineering and application of an expanded palette of ddFPs, which includes green (ddGFP) and yellow (ddYFP) variants. These optimized variants offer several advantages relative to ddRFP including increased in vitro contrast and brightness for ddGFP and increased brightness and a lowered pK a for ddYFP. We demonstrate that both variants are useful as biosensors for protease activity in live cells. Using the ddGFP tool, we generated a highly effective indicator of endomembrane proximity that can be used to image the mitochondria-associated membrane (MAM) interface of endoplasmic reticulum (ER) and mitochondria. Twit Text FOAVip Dimerization-dependent green and yellow fluorescent proteins ????ACS Synth Biol http://www.kidney.de/pget.php?&tw=1&pmid=23656278 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23656278 #FOAvip English Wikipedia <ref>{{Cite pmid|23656278}}</ref> Dimerization-dependent green and yellow fluorescent proteins #MMPMID23656278 Alford SC; Ding Y; Simmen T; Campbell RE ACS Synth Biol 2012[Dec]; 1 (12): 569-75 PMID23656278show ga Dimerization-dependent fluorescent proteins (ddFP) are a recently introduced class of genetically encoded reporters that can be used for the detection of protein interactions in live cells. The progenitor of this class of tools was a red fluorescent ddFP (ddRFP) derived from a homodimeric variant of Discosoma red fluorescent protein. Here, we describe the engineering and application of an expanded palette of ddFPs, which includes green (ddGFP) and yellow (ddYFP) variants. These optimized variants offer several advantages relative to ddRFP including increased in vitro contrast and brightness for ddGFP and increased brightness and a lowered pK a for ddYFP. We demonstrate that both variants are useful as biosensors for protease activity in live cells. Using the ddGFP tool, we generated a highly effective indicator of endomembrane proximity that can be used to image the mitochondria-associated membrane (MAM) interface of endoplasmic reticulum (ER) and mitochondria. |Bacterial Proteins/analysis/*chemistry/*metabolism[MESH] |Biosensing Techniques/methods[MESH] |Endoplasmic Reticulum/metabolism[MESH] |Fluorescent Antibody Technique/*methods[MESH] |Green Fluorescent Proteins/analysis/*chemistry/*metabolism[MESH] |Humans[MESH] |Luminescent Proteins/analysis/*chemistry/*metabolism[MESH] |Mitochondria/metabolism[MESH] |Mitochondrial Membranes/metabolism[MESH]Dimerization-dependent green and yellow fluorescent proteins (epmc).pdf DeepDyve Pubget Overpricing