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10.1016/j.toxlet.2013.01.015 http://scihub22266oqcxt.onion/10.1016/j.toxlet.2013.01.015 23402939!ä!23402939 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Toxicol+Lett 2013 ; 218 (3): 200-6 Nephropedia Template TP {{tp|p=23402939|t=2013. The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells |pdf=|usr=}}{{23402939}} gab.com Text The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells JO: Toxicol Lett http://www.kidney.de/pget.php?&tw=1&pmid=23402939 #FOAvip Effects of 96 h aflatoxin B1 (AFB1) exposure at concentrations from 0.2 muM to 6 muM on the mRNA and protein expression levels of the following transporters ABCB1/B4, ABCC1, ABCC2, ABCG2, OAT4 and the mRNA expression of steroid-metabolizing enzymes CYP1A1, CYP19A1, HSD3B1 and HSD17B1, and conjugating enzyme family UGT1A were evaluated in trophoblastic JEG-3 cells. Statistically significant dose-dependent five-fold increases in the expression levels with ABCC2 and OAT4 were recorded at 2 and 6muM AFB1. Protein expression of ABCG2 was decreased dose-dependently with 0.2-6 muM AFB1. With the other transporters, only a trend of increased expression was observed. Analogously, a three-fold increase in the expressions of CYP19A1, HSD3B1, HSD17B1 and UGT1A-family were observed at 0.3 muM AFB1. When an inhibitor of CYP19A1, finrozole, was dosed simultaneously with AFB1, no increases in the transcripts of transporters or steroid hydroxylases or CYP19A1 were observed. This delayed increase in the expression levels - only after 96h incubations - may indicate that the response is due to a secondary metabolite of AFB1 or other secondary controlling cascades rather than the parent compound itself. In conclusion, AFB1 affected the placental steroid synthesizing, metabolizing and conjugating enzymes as well as the expression levels of several transporter proteins in JEG-3 cells. These alterations may lead to anomalies in the foetoplacental hormonal homeostasis. Twit Text FOAVip The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells ????Toxicol Lett http://www.kidney.de/pget.php?&tw=1&pmid=23402939 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23402939 #FOAvip English Wikipedia <ref>{{Cite pmid|23402939}}</ref> The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells #MMPMID23402939 Huuskonen P; Myllynen P; Storvik M; Pasanen M Toxicol Lett 2013[Apr]; 218 (3): 200-6 PMID23402939show ga Effects of 96 h aflatoxin B1 (AFB1) exposure at concentrations from 0.2 muM to 6 muM on the mRNA and protein expression levels of the following transporters ABCB1/B4, ABCC1, ABCC2, ABCG2, OAT4 and the mRNA expression of steroid-metabolizing enzymes CYP1A1, CYP19A1, HSD3B1 and HSD17B1, and conjugating enzyme family UGT1A were evaluated in trophoblastic JEG-3 cells. Statistically significant dose-dependent five-fold increases in the expression levels with ABCC2 and OAT4 were recorded at 2 and 6muM AFB1. Protein expression of ABCG2 was decreased dose-dependently with 0.2-6 muM AFB1. With the other transporters, only a trend of increased expression was observed. Analogously, a three-fold increase in the expressions of CYP19A1, HSD3B1, HSD17B1 and UGT1A-family were observed at 0.3 muM AFB1. When an inhibitor of CYP19A1, finrozole, was dosed simultaneously with AFB1, no increases in the transcripts of transporters or steroid hydroxylases or CYP19A1 were observed. This delayed increase in the expression levels - only after 96h incubations - may indicate that the response is due to a secondary metabolite of AFB1 or other secondary controlling cascades rather than the parent compound itself. In conclusion, AFB1 affected the placental steroid synthesizing, metabolizing and conjugating enzymes as well as the expression levels of several transporter proteins in JEG-3 cells. These alterations may lead to anomalies in the foetoplacental hormonal homeostasis. |ATP Binding Cassette Transporter, Subfamily B, Member 1/drug effects/metabolism[MESH] |ATP Binding Cassette Transporter, Subfamily B/drug effects/metabolism[MESH] |ATP Binding Cassette Transporter, Subfamily G, Member 2[MESH] |ATP-Binding Cassette Transporters/*drug effects/genetics/metabolism[MESH] |Aflatoxin B1/*pharmacology[MESH] |Aromatase/metabolism[MESH] |Cell Line, Tumor[MESH] |Choriocarcinoma/*enzymology/genetics[MESH] |Cytochrome P-450 CYP1A1/metabolism[MESH] |Dose-Response Relationship, Drug[MESH] |Enzymes/genetics/*metabolism[MESH] |Estradiol Dehydrogenases/metabolism[MESH] |Gene Expression Regulation, Enzymologic[MESH] |Glucuronosyltransferase/metabolism[MESH] |Humans[MESH] |Multidrug Resistance-Associated Protein 2[MESH] |Multidrug Resistance-Associated Proteins/drug effects/metabolism[MESH] |Multienzyme Complexes/metabolism[MESH] |Neoplasm Proteins/drug effects/metabolism[MESH] |Organic Anion Transporters, Sodium-Independent/*drug effects/genetics/metabolism[MESH] |Progesterone Reductase/metabolism[MESH] |RNA, Messenger/metabolism[MESH] |Steroid Isomerases/metabolism[MESH] |Steroids/*biosynthesis[MESH]The effects of aflatoxin B1 on transporters and steroid metabolizing e(epmc).pdf DeepDyve Pubget Overpricing