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10.1002/jmv.23465

http://scihub22266oqcxt.onion/10.1002/jmv.23465
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23161446!7166784!23161446
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suck abstract from ncbi


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pmid23161446      J+Med+Virol 2013 ; 85 (2): 309-14
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  • Prevalence and molecular epidemiology of human coronavirus HKU1 in patients with acute respiratory illness #MMPMID23161446
  • Lee WJ; Chung YS; Yoon HS; Kang C; Kim K
  • J Med Virol 2013[Feb]; 85 (2): 309-14 PMID23161446show ga
  • In 2005, human coronavirus HKU1 (HCoV-HKU1) was isolated and identified from a 71-year-old man with pneumonia in Hong Kong. To identify and classify genotypes of HCoV-HKU1 in Korea, a sensitive, specific, and quantitative real-time polymerase chain reaction (PCR) assay was developed and analyzed the sequences of HCoV-HKU1 isolated in Korea. A total of 1,985 respiratory specimens taken from patients with acute respiratory illness were tested for HCoV-HKU1 from January 2007 to May 2008. The major clinical symptoms associated with HCoV-HKU1 infection were examined statistically and sequence variations of the RNA-dependent RNA polymerase (RdRp), spike, and nucleocapsid genes were also analyzed. Fifty cases (2.5%) HCoV-HKU1 were identified by real-time PCR and viral loads ranged from 6.7 x 10(4) to 1.6 x 10(9) copies/ml. The clinical symptoms of HCoV-HKU1 infection included rhinorrhea (72%), cough (64%), nasal congestion (56%), fever (32%), sputum (30%), sore throat (18%), chills (16%), postnasal discharge (14%), and tonsillar hypertrophy (10%). There was a seasonal distribution of HCoV-HKU1 infection, peaking in winter and spring. Both genotypes A and B were detected but no recombination between them was found. This is the first report on the identification and genotyping of HCoV-HKU1 as a causative agent of acute respiratory illness in Korea. The data suggest that at least two genotypes, A and B, of HCoV-HKU1 with scattered silent mutations were circulating in Korea from 2007 to 2008.
  • |Coronavirus Infections/*epidemiology/pathology/*virology[MESH]
  • |Coronavirus/*classification/genetics/*isolation & purification[MESH]
  • |Genotype[MESH]
  • |Humans[MESH]
  • |Korea/epidemiology[MESH]
  • |Membrane Glycoproteins/genetics[MESH]
  • |Molecular Epidemiology[MESH]
  • |Molecular Sequence Data[MESH]
  • |Nucleocapsid/genetics[MESH]
  • |RNA, Viral/genetics[MESH]
  • |RNA-Dependent RNA Polymerase/genetics[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |Respiratory Tract Infections/*epidemiology/pathology/*virology[MESH]
  • |Seasons[MESH]
  • |Sequence Analysis, DNA[MESH]
  • |Spike Glycoprotein, Coronavirus[MESH]
  • |Viral Envelope Proteins/genetics[MESH]


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