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http://scihub22266oqcxt.onion/ 23047499!ä!23047499 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Eur+Rev+Med+Pharmacol+Sci 2012 ; 16 (9): 1172-8 Nephropedia Template TP {{tp|p=23047499|t=2012. Retrovirus-mediated siRNA targeting TRPM7 gene induces apoptosis in RBL-2H3 cells |pdf=|usr=}}{{23047499}} gab.com Text Retrovirus-mediated siRNA targeting TRPM7 gene induces apoptosis in RBL-2H3 cells JO: Eur Rev Med Pharmacol Sci http://www.kidney.de/pget.php?&tw=1&pmid=23047499 #FOAvip BACKGROUND AND OBJECTIVE: Calcium signaling is important for both normal physiologic processes and pathology of various diseases. Transient receptor potential melastatin 7 (TRPM7) gene has been reported to be a potential candidate for calcium influx. The present study aimed to investigate the possible role of TRPM7 channels in apoptosis in rat basophilic leukemia mast cell line (RBL-2H3), which is widely used in mast cell-associated studies. MATERIALS AND METHODS: A recombinant retrovirus vector siRNA targeting rat TRPM7 gene was constructed and identified. Cellular survival was assessed by MTT. Cell apoptosis was evaluated by flow cytometry and TUNEL-FITC/Hoechst 33258 staining. RESULTS: The transfection efficiency by retrovirus vector was about 60%-70%. Transfection with TRPM7 siRNA significantly reduced TRPM7 expression both at mRNA and protein levels. Suppression of TRPM7 expression by siRNA led to significantly decreased cellular survival rates and increased apoptosis rates in RBL-2H3 cells. CONCLUSIONS: This study indicates that TRPM7 is involved in the apoptosis process in RBL-2H3 cells. Twit Text FOAVip Retrovirus-mediated siRNA targeting TRPM7 gene induces apoptosis in RBL-2H3 cells ????Eur Rev Med Pharmacol Sci http://www.kidney.de/pget.php?&tw=1&pmid=23047499 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23047499 #FOAvip English Wikipedia <ref>{{Cite pmid|23047499}}</ref> Retrovirus-mediated siRNA targeting TRPM7 gene induces apoptosis in RBL-2H3 cells #MMPMID23047499 Ng NM; Jiang SP; Lv ZQ Eur Rev Med Pharmacol Sci 2012[Sep]; 16 (9): 1172-8 PMID23047499show ga BACKGROUND AND OBJECTIVE: Calcium signaling is important for both normal physiologic processes and pathology of various diseases. Transient receptor potential melastatin 7 (TRPM7) gene has been reported to be a potential candidate for calcium influx. The present study aimed to investigate the possible role of TRPM7 channels in apoptosis in rat basophilic leukemia mast cell line (RBL-2H3), which is widely used in mast cell-associated studies. MATERIALS AND METHODS: A recombinant retrovirus vector siRNA targeting rat TRPM7 gene was constructed and identified. Cellular survival was assessed by MTT. Cell apoptosis was evaluated by flow cytometry and TUNEL-FITC/Hoechst 33258 staining. RESULTS: The transfection efficiency by retrovirus vector was about 60%-70%. Transfection with TRPM7 siRNA significantly reduced TRPM7 expression both at mRNA and protein levels. Suppression of TRPM7 expression by siRNA led to significantly decreased cellular survival rates and increased apoptosis rates in RBL-2H3 cells. CONCLUSIONS: This study indicates that TRPM7 is involved in the apoptosis process in RBL-2H3 cells. |*Apoptosis[MESH] |Animals[MESH] |Cell Line, Tumor[MESH] |Cell Survival[MESH] |Mast Cells/*physiology[MESH] |RNA, Small Interfering/*genetics[MESH] |Rats[MESH] |Retroviridae/*genetics[MESH]Retrovirus-mediated siRNA targeting TRPM7 gene induces apoptosis in RB(epmc).pdf DeepDyve Pubget Overpricing