Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1074/jbc.M112.386425 http://scihub22266oqcxt.onion/10.1074/jbc.M112.386425 22977238!3488056!22977238     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 2012 ; 287 (45): 37824-34 Nephropedia Template TP {{tp|p=22977238|t=2012. Dynamin2, clathrin, and lipid rafts mediate endocytosis of the apical Na/K/2Cl cotransporter NKCC2 in thick ascending limbs |pdf=|usr=}}{{22977238}} gab.com Text Dynamin2, clathrin, and lipid rafts mediate endocytosis of the apical Na/K/2Cl cotransporter NKCC2 in thick ascending limbs JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=22977238 #FOAvip Steady-state surface levels of the apical Na/K/2Cl cotransporter NKCC2 regulate NaCl reabsorption by epithelial cells of the renal thick ascending limb (THAL). We reported that constitutive endocytosis of NKCC2 controls NaCl absorption in native THALs; however, the pathways involved in NKCC2 endocytosis are unknown. We hypothesized that NKCC2 endocytosis at the apical surface depends on dynamin-2 and clathrin. Measurements of steady-state surface NKCC2 and the rate of NKCC2 endocytosis in freshly isolated rat THALs showed that inhibition of endogenous dynamin-2 with dynasore blunted NKCC2 endocytosis by 56 +/- 11% and increased steady-state surface NKCC2 by 67 +/- 27% (p < 0.05). Expression of the dominant negative Dyn2K44A in THALs slowed the rate of NKCC2 endocytosis by 38 +/- 8% and increased steady-state surface NKCC2 by 37 +/- 8%, without changing total NKCC2 expression. Inhibition of clathrin-mediated endocytosis with chlorpromazine blunted NKCC2 endocytosis by 54 +/- 6%, while preventing clathrin from interacting with synaptojanin also blunted NKCC2 endocytosis by 52 +/- 5%. Disruption of lipid rafts blunted NKCC2 endocytosis by 39 +/- 4% and silencing caveolin-1 by 29 +/- 4%. Simultaneous inhibition of clathrin- and lipid raft-mediated endocytosis completely blocked NKCC2 internalization. We concluded that dynamin-2, clathrin, and lipid rafts mediate NKCC2 endocytosis and maintain steady-state apical surface NKCC2 in native THALs. These are the first data identifying the endocytic pathway for apical NKCC2 endocytosis. Twit Text FOAVip Dynamin2, clathrin, and lipid rafts mediate endocytosis of the apical Na/K/2Cl cotransporter NKCC2 in thick ascending limbs ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=22977238 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22977238 #FOAvip English Wikipedia <ref>{{Cite pmid|22977238}}</ref> Dynamin2, clathrin, and lipid rafts mediate endocytosis of the apical Na/K/2Cl cotransporter NKCC2 in thick ascending limbs #MMPMID22977238 Ares GR; Ortiz PA J Biol Chem 2012[Nov]; 287 (45): 37824-34 PMID22977238show ga Steady-state surface levels of the apical Na/K/2Cl cotransporter NKCC2 regulate NaCl reabsorption by epithelial cells of the renal thick ascending limb (THAL). We reported that constitutive endocytosis of NKCC2 controls NaCl absorption in native THALs; however, the pathways involved in NKCC2 endocytosis are unknown. We hypothesized that NKCC2 endocytosis at the apical surface depends on dynamin-2 and clathrin. Measurements of steady-state surface NKCC2 and the rate of NKCC2 endocytosis in freshly isolated rat THALs showed that inhibition of endogenous dynamin-2 with dynasore blunted NKCC2 endocytosis by 56 +/- 11% and increased steady-state surface NKCC2 by 67 +/- 27% (p < 0.05). Expression of the dominant negative Dyn2K44A in THALs slowed the rate of NKCC2 endocytosis by 38 +/- 8% and increased steady-state surface NKCC2 by 37 +/- 8%, without changing total NKCC2 expression. Inhibition of clathrin-mediated endocytosis with chlorpromazine blunted NKCC2 endocytosis by 54 +/- 6%, while preventing clathrin from interacting with synaptojanin also blunted NKCC2 endocytosis by 52 +/- 5%. Disruption of lipid rafts blunted NKCC2 endocytosis by 39 +/- 4% and silencing caveolin-1 by 29 +/- 4%. Simultaneous inhibition of clathrin- and lipid raft-mediated endocytosis completely blocked NKCC2 internalization. We concluded that dynamin-2, clathrin, and lipid rafts mediate NKCC2 endocytosis and maintain steady-state apical surface NKCC2 in native THALs. These are the first data identifying the endocytic pathway for apical NKCC2 endocytosis. |*Endocytosis[MESH] |Animals[MESH] |Blotting, Western[MESH] |Caveolin 1/genetics/metabolism[MESH] |Chlorpromazine/pharmacology[MESH] |Clathrin Heavy Chains/antagonists & inhibitors/*metabolism[MESH] |Dynamin II/antagonists & inhibitors/genetics/*metabolism[MESH] |Hydrazones/pharmacology[MESH] |Loop of Henle/drug effects/*metabolism[MESH] |Male[MESH] |Membrane Microdomains/*metabolism[MESH] |Microscopy, Confocal[MESH] |Microscopy, Fluorescence[MESH] |Mutation[MESH] |RNA Interference[MESH] |Rats[MESH] |Rats, Sprague-Dawley[MESH] |Signal Transduction/drug effects[MESH] |Sodium-Potassium-Chloride Symporters/*metabolism[MESH]Dynamin2, clathrin, and lipid rafts mediate endocytosis of the apical (epmc).pdf DeepDyve Pubget Overpricing