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10.1080/10286020.2012.702108 http://scihub22266oqcxt.onion/10.1080/10286020.2012.702108 22924574!ä!22924574 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Asian+Nat+Prod+Res 2012 ; 14 (9): 877-85 Nephropedia Template TP {{tp|p=22924574|t=2012. Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway |pdf=|usr=}}{{22924574}} gab.com Text Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway JO: J Asian Nat Prod Res http://www.kidney.de/pget.php?&tw=1&pmid=22924574 #FOAvip The antivirus effect of quercetin and oseltamivir on the Toll-like receptor 7 (TLR7) signaling pathway was observed when dendritic cells and macrophages were infected with H1N1. Leukomonocytes were obtained from umbilical cord blood and harvested after stimulation by recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (rhGM-CSF) and recombinant human Interleukin 4 (rhIL-4). Virus-infected cell model was established by human bronchial epithelial cells (16HBE) infected with H1N1. After immunological cells and virus-infected cells were co-cultured, quercetin and oseltamivir were also added into the medium as a treatment intervention. Then the immunological cells were collected for Real Time PCR (RT-PCR) and Western blot to determine the expression levels of genes related to TLR7 pathway. Viral infection led to cell death and increased the gene expression levels of TLR7 signal pathway. Quercetin and oseltamivir increased cell viability and reduced the expression levels of TLR7 signal pathway. Twit Text FOAVip Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway ????J Asian Nat Prod Res http://www.kidney.de/pget.php?&tw=1&pmid=22924574 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22924574 #FOAvip English Wikipedia <ref>{{Cite pmid|22924574}}</ref> Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway #MMPMID22924574 Chen C; Jiang ZY; Yu B; Wu XL; Dai CQ; Zhao CL; Ju DH; Chen XY J Asian Nat Prod Res 2012[]; 14 (9): 877-85 PMID22924574show ga The antivirus effect of quercetin and oseltamivir on the Toll-like receptor 7 (TLR7) signaling pathway was observed when dendritic cells and macrophages were infected with H1N1. Leukomonocytes were obtained from umbilical cord blood and harvested after stimulation by recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (rhGM-CSF) and recombinant human Interleukin 4 (rhIL-4). Virus-infected cell model was established by human bronchial epithelial cells (16HBE) infected with H1N1. After immunological cells and virus-infected cells were co-cultured, quercetin and oseltamivir were also added into the medium as a treatment intervention. Then the immunological cells were collected for Real Time PCR (RT-PCR) and Western blot to determine the expression levels of genes related to TLR7 pathway. Viral infection led to cell death and increased the gene expression levels of TLR7 signal pathway. Quercetin and oseltamivir increased cell viability and reduced the expression levels of TLR7 signal pathway. |Blotting, Western[MESH] |Cell Survival/drug effects[MESH] |Dendritic Cells/drug effects[MESH] |Granulocyte Colony-Stimulating Factor/pharmacology[MESH] |Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology[MESH] |Humans[MESH] |Influenza A Virus, H1N1 Subtype/*drug effects[MESH] |Interleukin-3/pharmacology[MESH] |Interleukin-4/pharmacology[MESH] |Oseltamivir/*pharmacology[MESH] |Quercetin/*pharmacology[MESH] |Recombinant Proteins[MESH]Study on the anti-H1N1 virus effects of quercetin and oseltamivir and (epmc).pdf DeepDyve Pubget Overpricing