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10.1152/ajprenal.00023.2012 http://scihub22266oqcxt.onion/10.1152/ajprenal.00023.2012 22791339!3468522!22791339     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol+Renal+Physiol 2012 ; 303 (6): F821-30 Nephropedia Template TP {{tp|p=22791339|t=2012. Endotoxemia alters tight junction gene and protein expression in the kidney |pdf=|usr=}}{{22791339}} gab.com Text Endotoxemia alters tight junction gene and protein expression in the kidney JO: Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=22791339 #FOAvip Intact tight junctional (TJ) proteins are required for tubular ion transport and waste excretion. Disruption of TJs may contribute to a decreased glomerular filtration rate in acute kidney injury (AKI) via tubular backleak. The effect of LPS-mediated AKI on murine TJs has not been studied extensively. We hypothesized LPS endotoxin administration to mice would disrupt tubular TJ proteins including zonula occludens-1 (ZO-1), occludin, and claudins. ZO-1 and occludin immunofluorescence 24 h post-LPS revealed a marked change in localization from the usual circumferential fencework pattern to one with substantial fragmentation. Renal ZO-1 expression was significantly reduced 24 h after LPS (decrease of 56.1 +/- 7.4%, P < 0.001), with subsequent recovery. ZO-1 mRNA expression was increased 24 h post-LPS (4.34 +/- 0.87-fold, P = 0.0019), suggesting disruption of ZO-1 protein is not mediated by transcriptional regulation, but rather by degradation or changes in translation. Similarly, claudin-4 protein expression was decreased despite elevated mRNA. LPS administration resulted in dephosphorylation of occludin and fragmented tubular redistribution. Protein expression of claudin-1, and -3 was increased after LPS. ZO-1, occludin, and claudin-1, -3, and -4 gene expression were increased 48 h after LPS, suggesting a renal response to strengthen TJs following injury. Interestingly, reduced mRNA expression was found only for claudin-8. This study provides further support that LPS-induced AKI is associated with structural injury and is not merely due to hemodynamic changes. Twit Text FOAVip Endotoxemia alters tight junction gene and protein expression in the kidney ????Am J Physiol Renal Physiol http://www.kidney.de/pget.php?&tw=1&pmid=22791339 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22791339 #FOAvip English Wikipedia <ref>{{Cite pmid|22791339}}</ref> Endotoxemia alters tight junction gene and protein expression in the kidney #MMPMID22791339 Eadon MT; Hack BK; Xu C; Ko B; Toback FG; Cunningham PN Am J Physiol Renal Physiol 2012[Sep]; 303 (6): F821-30 PMID22791339show ga Intact tight junctional (TJ) proteins are required for tubular ion transport and waste excretion. Disruption of TJs may contribute to a decreased glomerular filtration rate in acute kidney injury (AKI) via tubular backleak. The effect of LPS-mediated AKI on murine TJs has not been studied extensively. We hypothesized LPS endotoxin administration to mice would disrupt tubular TJ proteins including zonula occludens-1 (ZO-1), occludin, and claudins. ZO-1 and occludin immunofluorescence 24 h post-LPS revealed a marked change in localization from the usual circumferential fencework pattern to one with substantial fragmentation. Renal ZO-1 expression was significantly reduced 24 h after LPS (decrease of 56.1 +/- 7.4%, P < 0.001), with subsequent recovery. ZO-1 mRNA expression was increased 24 h post-LPS (4.34 +/- 0.87-fold, P = 0.0019), suggesting disruption of ZO-1 protein is not mediated by transcriptional regulation, but rather by degradation or changes in translation. Similarly, claudin-4 protein expression was decreased despite elevated mRNA. LPS administration resulted in dephosphorylation of occludin and fragmented tubular redistribution. Protein expression of claudin-1, and -3 was increased after LPS. ZO-1, occludin, and claudin-1, -3, and -4 gene expression were increased 48 h after LPS, suggesting a renal response to strengthen TJs following injury. Interestingly, reduced mRNA expression was found only for claudin-8. This study provides further support that LPS-induced AKI is associated with structural injury and is not merely due to hemodynamic changes. |Acute Disease[MESH] |Animals[MESH] |Endotoxemia/chemically induced/genetics/*metabolism[MESH] |Gene Expression Regulation[MESH] |Kidney/*metabolism/pathology[MESH] |Lipopolysaccharides/toxicity[MESH] |Male[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Tight Junction Proteins/genetics/*metabolism[MESH]Endotoxemia alters tight junction gene and protein expression in the k(epmc).pdf DeepDyve Pubget Overpricing