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10.1126/science.1225829 http://scihub22266oqcxt.onion/10.1126/science.1225829 22745249!6286148!22745249     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Science 2012 ; 337 (6096): 816-21 Nephropedia Template TP {{tp|p=22745249|t=2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity |pdf=|usr=}}{{22745249}} gab.com Text A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=22745249 #FOAvip Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subset of these systems, the mature crRNA that is base-paired to trans-activating crRNA (tracrRNA) forms a two-RNA structure that directs the CRISPR-associated protein Cas9 to introduce double-stranded (ds) breaks in target DNA. At sites complementary to the crRNA-guide sequence, the Cas9 HNH nuclease domain cleaves the complementary strand, whereas the Cas9 RuvC-like domain cleaves the noncomplementary strand. The dual-tracrRNA:crRNA, when engineered as a single RNA chimera, also directs sequence-specific Cas9 dsDNA cleavage. Our study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing. Twit Text FOAVip A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity ????Science http://www.kidney.de/pget.php?&tw=1&pmid=22745249 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22745249 #FOAvip English Wikipedia <ref>{{Cite pmid|22745249}}</ref> A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity #MMPMID22745249 Jinek M; Chylinski K; Fonfara I; Hauer M; Doudna JA; Charpentier E Science 2012[Aug]; 337 (6096): 816-21 PMID22745249show ga Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subset of these systems, the mature crRNA that is base-paired to trans-activating crRNA (tracrRNA) forms a two-RNA structure that directs the CRISPR-associated protein Cas9 to introduce double-stranded (ds) breaks in target DNA. At sites complementary to the crRNA-guide sequence, the Cas9 HNH nuclease domain cleaves the complementary strand, whereas the Cas9 RuvC-like domain cleaves the noncomplementary strand. The dual-tracrRNA:crRNA, when engineered as a single RNA chimera, also directs sequence-specific Cas9 dsDNA cleavage. Our study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing. |*DNA Breaks, Double-Stranded[MESH] |*DNA Cleavage[MESH] |*Inverted Repeat Sequences[MESH] |Bacteriophages/*immunology[MESH] |Base Sequence[MESH] |Deoxyribonucleases, Type II Site-Specific/chemistry/genetics/*metabolism[MESH] |Molecular Sequence Data[MESH] |Nucleic Acid Conformation[MESH] |Plasmids/metabolism[MESH] |RNA/chemistry/*metabolism[MESH]A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial (epmc).pdf DeepDyve Pubget Overpricing