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10.1128/JVI.01004-12 http://scihub22266oqcxt.onion/10.1128/JVI.01004-12 22718826!3416153!22718826     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol 2012 ; 86 (17): 9274-84 Nephropedia Template TP {{tp|p=22718826|t=2012. Characterization of the early steps of human parvovirus B19 infection |pdf=|usr=}}{{22718826}} gab.com Text Characterization of the early steps of human parvovirus B19 infection JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=22718826 #FOAvip The early steps of human parvovirus B19 (B19V) infection were investigated in UT7/Epo cells. B19V and its receptor globoside (Gb4Cer) associate with lipid rafts, predominantly of the noncaveolar type. Pharmacological disruption of the lipid rafts inhibited infection when the drug was added prior to virus attachment but not after virus uptake. B19V is internalized by clathrin-dependent endocytosis and spreads rapidly throughout the endocytic pathway, reaching the lysosomal compartment within minutes, where a substantial proportion is degraded. B19V did not permeabilize the endocytic vesicles, indicating a mechanism of endosomal escape without apparent membrane damage. Bafilomycin A(1) (BafA1) and NH(4)Cl, which raise endosomal pH, blocked the infection by preventing endosomal escape, resulting in a massive accumulation of capsids in the lysosomes. In contrast, in the presence of chloroquine (CQ), the transfer of incoming viruses from late endosomes to lysosomes was prevented; the viral DNA was not degraded; and the infection was boosted. In contrast to the findings for untreated or BafA1-treated cells, the viral DNA was progressively associated with the nucleus in CQ-treated cells, reaching a plateau by 3 h postinternalization, a time coinciding with the initiation of viral transcription. At this time, more than half of the total intracellular viral DNA was associated with the nucleus; however, the capsids remained extranuclear. Our studies provide the first insight into the early steps of B19V infection and reveal mechanisms involved in virus uptake, endocytic trafficking, and nuclear penetration. Twit Text FOAVip Characterization of the early steps of human parvovirus B19 infection ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=22718826 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22718826 #FOAvip English Wikipedia <ref>{{Cite pmid|22718826}}</ref> Characterization of the early steps of human parvovirus B19 infection #MMPMID22718826 Quattrocchi S; Ruprecht N; Bonsch C; Bieli S; Zurcher C; Boller K; Kempf C; Ros C J Virol 2012[Sep]; 86 (17): 9274-84 PMID22718826show ga The early steps of human parvovirus B19 (B19V) infection were investigated in UT7/Epo cells. B19V and its receptor globoside (Gb4Cer) associate with lipid rafts, predominantly of the noncaveolar type. Pharmacological disruption of the lipid rafts inhibited infection when the drug was added prior to virus attachment but not after virus uptake. B19V is internalized by clathrin-dependent endocytosis and spreads rapidly throughout the endocytic pathway, reaching the lysosomal compartment within minutes, where a substantial proportion is degraded. B19V did not permeabilize the endocytic vesicles, indicating a mechanism of endosomal escape without apparent membrane damage. Bafilomycin A(1) (BafA1) and NH(4)Cl, which raise endosomal pH, blocked the infection by preventing endosomal escape, resulting in a massive accumulation of capsids in the lysosomes. In contrast, in the presence of chloroquine (CQ), the transfer of incoming viruses from late endosomes to lysosomes was prevented; the viral DNA was not degraded; and the infection was boosted. In contrast to the findings for untreated or BafA1-treated cells, the viral DNA was progressively associated with the nucleus in CQ-treated cells, reaching a plateau by 3 h postinternalization, a time coinciding with the initiation of viral transcription. At this time, more than half of the total intracellular viral DNA was associated with the nucleus; however, the capsids remained extranuclear. Our studies provide the first insight into the early steps of B19V infection and reveal mechanisms involved in virus uptake, endocytic trafficking, and nuclear penetration. |Cell Line[MESH] |Cell Nucleus/virology[MESH] |Endocytosis[MESH] |Endosomes/virology[MESH] |Erythema Infectiosum/metabolism/*virology[MESH] |Globosides/metabolism[MESH] |Humans[MESH] |Lysosomes/virology[MESH] |Parvovirus B19, Human/*physiology[MESH]Characterization of the early steps of human parvovirus B19 infection (epmc).pdf DeepDyve Pubget Overpricing