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10.1002/jmv.23281 http://scihub22266oqcxt.onion/10.1002/jmv.23281 22499014!7166974!22499014     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2012 ; 84 (6): 906-11 Nephropedia Template TP {{tp|p=22499014|t=2012. Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens |pdf=|usr=}}{{22499014}} gab.com Text Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=22499014 #FOAvip The aim of the study was to evaluate the MagNA Pure 96 nucleic acid extraction system using clinical respiratory specimens for identifying viruses by qualitative real-time PCR assays. Three extraction methods were tested, that is, the MagNA Pure LC, the COBAS Ampliprep, and the MagNA Pure 96 with 10-fold dilutions of an influenza A(H1N1)pdm09 sample. Two hundred thirty-nine respiratory specimens, 35 throat swabs, 164 nasopharyngeal specimens, and 40 broncho-alveolar fluids, were extracted with the MagNA Pure 96 and the COBAS Ampliprep instruments. Forty COBAS Ampliprep positive samples were also tested. Real-time PCRs were used to identify influenza A and influenza A(H1N1)pdm09, rhinovirus, enterovirus, adenovirus, varicella zoster virus, cytomegalovirus, and herpes simplex virus. Similar results were obtained on RNA extracted from dilutions of influenza A(H1N1)pdm09 with the three systems: the MagNA Pure LC, the COBAS Ampliprep, and the MagNA Pure 96. Data from clinical respiratory specimens extracted with the MagNA Pure 96 and COBAS Ampliprep instruments were in 98.5% in agreement (P < 0.0001) for influenza A and influenza A(H1N1)pdm09. Data for rhinovirus were in 97.3% agreement (P < 0.0001) and in 96.8% agreement for enterovirus. They were in 100% agreement for adenovirus. Data for cytomegalovirus and HSV1-2 were in 95.2% agreement (P < 0.0001). The MagNA Pure 96 instrument is easy-to-use, reliable, and has a high throughput for extracting total nucleic acid from respiratory specimens. These extracts are suitable for molecular diagnosis with any type of real-time PCR assay. Twit Text FOAVip Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=22499014 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22499014 #FOAvip English Wikipedia <ref>{{Cite pmid|22499014}}</ref> Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens #MMPMID22499014 Mengelle C; Mansuy JM; Sandres-Saune K; Barthe C; Boineau J; Izopet J J Med Virol 2012[Jun]; 84 (6): 906-11 PMID22499014show ga The aim of the study was to evaluate the MagNA Pure 96 nucleic acid extraction system using clinical respiratory specimens for identifying viruses by qualitative real-time PCR assays. Three extraction methods were tested, that is, the MagNA Pure LC, the COBAS Ampliprep, and the MagNA Pure 96 with 10-fold dilutions of an influenza A(H1N1)pdm09 sample. Two hundred thirty-nine respiratory specimens, 35 throat swabs, 164 nasopharyngeal specimens, and 40 broncho-alveolar fluids, were extracted with the MagNA Pure 96 and the COBAS Ampliprep instruments. Forty COBAS Ampliprep positive samples were also tested. Real-time PCRs were used to identify influenza A and influenza A(H1N1)pdm09, rhinovirus, enterovirus, adenovirus, varicella zoster virus, cytomegalovirus, and herpes simplex virus. Similar results were obtained on RNA extracted from dilutions of influenza A(H1N1)pdm09 with the three systems: the MagNA Pure LC, the COBAS Ampliprep, and the MagNA Pure 96. Data from clinical respiratory specimens extracted with the MagNA Pure 96 and COBAS Ampliprep instruments were in 98.5% in agreement (P < 0.0001) for influenza A and influenza A(H1N1)pdm09. Data for rhinovirus were in 97.3% agreement (P < 0.0001) and in 96.8% agreement for enterovirus. They were in 100% agreement for adenovirus. Data for cytomegalovirus and HSV1-2 were in 95.2% agreement (P < 0.0001). The MagNA Pure 96 instrument is easy-to-use, reliable, and has a high throughput for extracting total nucleic acid from respiratory specimens. These extracts are suitable for molecular diagnosis with any type of real-time PCR assay. |Automation/*methods[MESH] |Bronchoalveolar Lavage Fluid/virology[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/methods[MESH] |Nasopharynx/virology[MESH] |Nucleic Acids/*isolation & purification[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |Respiratory Tract Infections/*diagnosis/virology[MESH] |Sensitivity and Specificity[MESH] |Specimen Handling/*methods[MESH] |Virology/*methods[MESH] |Virus Diseases/*diagnosis/virology[MESH]Prospective evaluation of a new automated nucleic acid extraction syst(epmc).pdf DeepDyve Pubget Overpricing