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10.1016/j.jss.2012.02.012 http://scihub22266oqcxt.onion/10.1016/j.jss.2012.02.012 22487382!ä!22487382 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Surg+Res 2013 ; 179 (1): e189-95 Nephropedia Template TP {{tp|p=22487382|t=2013. Magnesium sulfate inhibits the secretion of high mobility group box 1 from lipopolysaccharide-activated RAW264 7 macrophages in vitro |pdf=|usr=}}{{22487382}} gab.com Text Magnesium sulfate inhibits the secretion of high mobility group box 1 from lipopolysaccharide-activated RAW264 7 macrophages in vitro JO: J Surg Res http://www.kidney.de/pget.php?&tw=1&pmid=22487382 #FOAvip BACKGROUND: High mobility group box 1 (HMGB1) is an important inflammatory factor that is closely related to mortality in patients with sepsis. High magnesium therapy has been proved to reduce sepsis-related mortality and sepsis-induced pathologic complications. These effects result from reduced expression and release of many inflammatory cytokines, although it is not clear whether high magnesium affects the expression and release of HMGB1. In the present study, we explored the effect of magnesium sulfate on the expression and release of HMGB1 in lipopolysaccharide (LPS)-activated macrophages. METHODS: RAW264.7 cells were incubated with LPS in the presence or absence of various concentrations of magnesium sulfate. An enzyme-linked immunosorbent assay was used to detect the levels of HMGB1 in the culture supernatant. Real-time polymerase chain reaction was used to assess the expression of HMGB1 mRNA. The nuclear/cytoplasm extraction kit was used to extract the nuclear and cytoplasmic proteins. Western blotting was used to observe the changes in the translocation of HMGB1 from the nucleus to the cytoplasm. The nuclear factor (NF)-kappaB p50/p65 Transcription Factor Assay Kit was used to analyze NF-kappaB activity in the nuclear extract. RESULTS: We found that magnesium sulfate inhibited translocation of HMGB1 from the nucleus to the cytoplasm and its extracellular release in LPS-activated macrophages and also suppressed the expression of HMGB1 mRNA. Furthermore, magnesium sulfate inhibited the translocation of NF-kappaB from the cytoplasm to the nucleus in LPS-activated macrophages in a dose-dependent manner. CONCLUSIONS: Our study has demonstrated that magnesium sulfate inhibits the translocation of HMGB1 from the nucleus to the cytoplasm and the expression of HMGB1 mRNA in a dose-dependent manner. The mechanism responsible for these effects involves the NF-kappaB signaling pathway. Twit Text FOAVip Magnesium sulfate inhibits the secretion of high mobility group box 1 from lipopolysaccharide-activated RAW264 7 macrophages in vitro ????J Surg Res http://www.kidney.de/pget.php?&tw=1&pmid=22487382 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22487382 #FOAvip English Wikipedia <ref>{{Cite pmid|22487382}}</ref> Magnesium sulfate inhibits the secretion of high mobility group box 1 from lipopolysaccharide-activated RAW264 7 macrophages in vitro #MMPMID22487382 Liu Z; Zhang J; Huang X; Huang L; Li S; Wang Z J Surg Res 2013[Jan]; 179 (1): e189-95 PMID22487382show ga BACKGROUND: High mobility group box 1 (HMGB1) is an important inflammatory factor that is closely related to mortality in patients with sepsis. High magnesium therapy has been proved to reduce sepsis-related mortality and sepsis-induced pathologic complications. These effects result from reduced expression and release of many inflammatory cytokines, although it is not clear whether high magnesium affects the expression and release of HMGB1. In the present study, we explored the effect of magnesium sulfate on the expression and release of HMGB1 in lipopolysaccharide (LPS)-activated macrophages. METHODS: RAW264.7 cells were incubated with LPS in the presence or absence of various concentrations of magnesium sulfate. An enzyme-linked immunosorbent assay was used to detect the levels of HMGB1 in the culture supernatant. Real-time polymerase chain reaction was used to assess the expression of HMGB1 mRNA. The nuclear/cytoplasm extraction kit was used to extract the nuclear and cytoplasmic proteins. Western blotting was used to observe the changes in the translocation of HMGB1 from the nucleus to the cytoplasm. The nuclear factor (NF)-kappaB p50/p65 Transcription Factor Assay Kit was used to analyze NF-kappaB activity in the nuclear extract. RESULTS: We found that magnesium sulfate inhibited translocation of HMGB1 from the nucleus to the cytoplasm and its extracellular release in LPS-activated macrophages and also suppressed the expression of HMGB1 mRNA. Furthermore, magnesium sulfate inhibited the translocation of NF-kappaB from the cytoplasm to the nucleus in LPS-activated macrophages in a dose-dependent manner. CONCLUSIONS: Our study has demonstrated that magnesium sulfate inhibits the translocation of HMGB1 from the nucleus to the cytoplasm and the expression of HMGB1 mRNA in a dose-dependent manner. The mechanism responsible for these effects involves the NF-kappaB signaling pathway. |Animals[MESH] |Cell Line[MESH] |Cell Nucleus/metabolism[MESH] |Cells, Cultured[MESH] |Cytoplasm/metabolism[MESH] |Dose-Response Relationship, Drug[MESH] |HMGB1 Protein/*metabolism[MESH] |In Vitro Techniques[MESH] |Lipopolysaccharides/*pharmacology[MESH] |Macrophage Activation/*drug effects/physiology[MESH] |Macrophages/cytology/*drug effects/*metabolism[MESH] |Magnesium Sulfate/*pharmacology[MESH] |Mice[MESH] |NF-kappa B/metabolism[MESH] |Protein Transport/drug effects/physiology[MESH] |RNA, Messenger/metabolism[MESH]Magnesium sulfate inhibits the secretion of high mobility group box 1 (epmc).pdf DeepDyve Pubget Overpricing