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10.1684/mrh.2012.0302 http://scihub22266oqcxt.onion/10.1684/mrh.2012.0302 22456185!ä!22456185 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Magnes+Res 2012 ; 25 (1): 12-20 Nephropedia Template TP {{tp|p=22456185|t=2012. Blocking the rise of intracellular calcium inhibits the growth of cells cultured in different concentrations of magnesium |pdf=|usr=}}{{22456185}} gab.com Text Blocking the rise of intracellular calcium inhibits the growth of cells cultured in different concentrations of magnesium JO: Magnes Res http://www.kidney.de/pget.php?&tw=1&pmid=22456185 #FOAvip Divalent cations, especially calcium and magnesium, have been shown to play an important regulatory role in endothelial and immune cells. To learn more about the interaction of these two metals in the regulation of cell growth, we altered the calcium/magnesium ratio by culturing human endothelial cells, macrophages, and T lymphocytes in media containing different concentrations of magnesium. We observed that the growth of the three cell types was retarded in low extracellular magnesium, and this retardation is particularly evident in highly proliferating cells. High concentrations of magnesium does not exert any effect on cell growth. When (i) calcium influx was blocked by adding the calcium antagonist verapamil, and (ii) calcium release from intracellular stores was inhibited by exposure to TMB-8, the growth of endothelial cells, macrophages, and T lymphocytes was inhibited. In particular, the release of calcium from intracellular stores seems to be more important than its influx in sustaining cell proliferation. Our results indicate that calcium plays a crucial role in mediating cell proliferation independently from the extracellular concentrations of magnesium. Twit Text FOAVip Blocking the rise of intracellular calcium inhibits the growth of cells cultured in different concentrations of magnesium ????Magnes Res http://www.kidney.de/pget.php?&tw=1&pmid=22456185 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22456185 #FOAvip English Wikipedia <ref>{{Cite pmid|22456185}}</ref> Blocking the rise of intracellular calcium inhibits the growth of cells cultured in different concentrations of magnesium #MMPMID22456185 Libako P; Castiglioni S; Baldoli E; Mazur A; Nowacki W; Maier JA Magnes Res 2012[Mar]; 25 (1): 12-20 PMID22456185show ga Divalent cations, especially calcium and magnesium, have been shown to play an important regulatory role in endothelial and immune cells. To learn more about the interaction of these two metals in the regulation of cell growth, we altered the calcium/magnesium ratio by culturing human endothelial cells, macrophages, and T lymphocytes in media containing different concentrations of magnesium. We observed that the growth of the three cell types was retarded in low extracellular magnesium, and this retardation is particularly evident in highly proliferating cells. High concentrations of magnesium does not exert any effect on cell growth. When (i) calcium influx was blocked by adding the calcium antagonist verapamil, and (ii) calcium release from intracellular stores was inhibited by exposure to TMB-8, the growth of endothelial cells, macrophages, and T lymphocytes was inhibited. In particular, the release of calcium from intracellular stores seems to be more important than its influx in sustaining cell proliferation. Our results indicate that calcium plays a crucial role in mediating cell proliferation independently from the extracellular concentrations of magnesium. |Animals[MESH] |Biological Transport/drug effects[MESH] |Calcium/*metabolism[MESH] |Cell Line[MESH] |Cell Proliferation/drug effects[MESH] |Gallic Acid/analogs & derivatives/pharmacology[MESH] |Magnesium/*pharmacology[MESH] |Mice[MESH]Blocking the rise of intracellular calcium inhibits the growth of cell(epmc).pdf DeepDyve Pubget Overpricing