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Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Acta+Crystallogr+Sect+F+Struct+Biol+Cryst+Commun 2011 ; 67 (Pt 11): 1359-62 Nephropedia Template TP
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Purification, crystallization and diffraction studies of the methyltransferases BT_2972 and BVU_3255 from antibiotic-resistant pathogens of the genus Bacteroides from the human intestine #MMPMID22102231
Kumar V; Mallika N; Sivaraman J
Acta Crystallogr Sect F Struct Biol Cryst Commun 2011[Nov]; 67 (Pt 11): 1359-62 PMID22102231show ga
The methyltransferases BT_2972 and BVU_3255 from two different Bacteroides species that are antibiotic-resistant pathogens from the human intestine were cloned, overexpressed and purified, yielding approximately 120 mg of each protein from 1 l culture. Apo BT_2972 and BVU_3255 and their complexes with S-adenosylmethionine or S-adenosylhomocysteine were crystallized in four different crystal forms using the hanging-drop vapour-diffusion method. These crystals diffracted to resolutions ranging from 2.8 to 2.2 A. Sequence analysis suggested that the two proteins are homologous small-molecule methyltransferases.