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10.1107/S1744309111031812

http://scihub22266oqcxt.onion/10.1107/S1744309111031812
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22102231/?report=reader!3212450!22102231
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suck abstract from ncbi


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pmid22102231      Acta+Crystallogr+Sect+F+Struct+Biol+Cryst+Commun 2011 ; 67 (Pt 11): 1359-62
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  • Purification, crystallization and diffraction studies of the methyltransferases BT_2972 and BVU_3255 from antibiotic-resistant pathogens of the genus Bacteroides from the human intestine #MMPMID22102231
  • Kumar V; Mallika N; Sivaraman J
  • Acta Crystallogr Sect F Struct Biol Cryst Commun 2011[Nov]; 67 (Pt 11): 1359-62 PMID22102231show ga
  • The methyltransferases BT_2972 and BVU_3255 from two different Bacteroides species that are antibiotic-resistant pathogens from the human intestine were cloned, overexpressed and purified, yielding approximately 120 mg of each protein from 1 l culture. Apo BT_2972 and BVU_3255 and their complexes with S-adenosylmethionine or S-adenosylhomocysteine were crystallized in four different crystal forms using the hanging-drop vapour-diffusion method. These crystals diffracted to resolutions ranging from 2.8 to 2.2 A. Sequence analysis suggested that the two proteins are homologous small-molecule methyltransferases.
  • |Bacteroides/*enzymology[MESH]
  • |Crystallization[MESH]
  • |Crystallography, X-Ray[MESH]
  • |Humans[MESH]
  • |Intestines/microbiology[MESH]


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