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10.1002/jmv.22186 http://scihub22266oqcxt.onion/10.1002/jmv.22186 22052551!7166524!22052551     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2012 ; 84 (1): 162-9 Nephropedia Template TP {{tp|p=22052551|t=2012. Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections |pdf=|usr=}}{{22052551}} gab.com Text Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=22052551 #FOAvip Respiratory viral infections are one of the leading causes of morbidity and mortality, particularly in children, the elderly and immunocompromised persons. Rapid identification of viral etiology is critical in ruling out non-viral infections, initiating antiviral treatment and limiting the spread of the infection. Multiplex assays of more than one viral gene target in a single tube have the advantage of rapid screening of a large number of potential viral pathogens in a short time. A multiplex real-time PCR assay was used in this study for detection of respiratory RNA and DNA viral infections in 728 specimens received from 585 adult and pediatric patients comprised of symptomatic and asymptomatic organ transplant recipients and non-recipients for diagnosis of respiratory illnesses and for routine clinical monitoring. Multiplex PCR was more sensitive than the multiplex immunofluoresence culture assay (R-mix) and also detected additional respiratory viruses that were not covered by the R-mix panel. The number of respiratory viruses detected in symptomatic patients was significantly higher than asymptomatic patients in both adult and pediatric patients. Herpesviral infections were the predominant cause of lower respiratory tract infection in the organ transplant recipients, whereas respiratory syncytial virus was the most common pathogen in non-transplant patients particularly children. Multiplex real-time PCR for detection of respiratory viruses has the potential for rapid identification of viral pathogens. In this era of emerging viral infections, addition of newer viral targets to the multiplex PCR panels will be beneficial in determining both patient management and public health epidemiology. Twit Text FOAVip Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=22052551 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22052551 #FOAvip English Wikipedia <ref>{{Cite pmid|22052551}}</ref> Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections #MMPMID22052551 Sanghavi SK; Bullotta A; Husain S; Rinaldo CR J Med Virol 2012[Jan]; 84 (1): 162-9 PMID22052551show ga Respiratory viral infections are one of the leading causes of morbidity and mortality, particularly in children, the elderly and immunocompromised persons. Rapid identification of viral etiology is critical in ruling out non-viral infections, initiating antiviral treatment and limiting the spread of the infection. Multiplex assays of more than one viral gene target in a single tube have the advantage of rapid screening of a large number of potential viral pathogens in a short time. A multiplex real-time PCR assay was used in this study for detection of respiratory RNA and DNA viral infections in 728 specimens received from 585 adult and pediatric patients comprised of symptomatic and asymptomatic organ transplant recipients and non-recipients for diagnosis of respiratory illnesses and for routine clinical monitoring. Multiplex PCR was more sensitive than the multiplex immunofluoresence culture assay (R-mix) and also detected additional respiratory viruses that were not covered by the R-mix panel. The number of respiratory viruses detected in symptomatic patients was significantly higher than asymptomatic patients in both adult and pediatric patients. Herpesviral infections were the predominant cause of lower respiratory tract infection in the organ transplant recipients, whereas respiratory syncytial virus was the most common pathogen in non-transplant patients particularly children. Multiplex real-time PCR for detection of respiratory viruses has the potential for rapid identification of viral pathogens. In this era of emerging viral infections, addition of newer viral targets to the multiplex PCR panels will be beneficial in determining both patient management and public health epidemiology. |Adolescent[MESH] |Adult[MESH] |Asymptomatic Diseases[MESH] |Child[MESH] |Humans[MESH] |Infant[MESH] |Infant, Newborn[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Multiplex Polymerase Chain Reaction/*methods[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |Respiratory Tract Infections/*virology[MESH] |Sensitivity and Specificity[MESH] |Virology/methods[MESH] |Virus Diseases/*diagnosis[MESH]Clinical evaluation of multiplex real-time PCR panels for rapid detect(epmc).pdf DeepDyve Pubget Overpricing