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10.1002/pmic.201100224 http://scihub22266oqcxt.onion/10.1002/pmic.201100224 21919203!7167899!21919203     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proteomics 2011 ; 11 (23): 4477-91 Nephropedia Template TP {{tp|p=21919203|t=2011. Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle |pdf=|usr=}}{{21919203}} gab.com Text Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle JO: Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=21919203 #FOAvip To identify host factors involved in Salmonella replication, SILAC-based quantitative proteomics was used to investigate the interactions of Salmonella typhimurium with the secretory pathway in human epithelial cells. Protein profiles of Golgi-enriched fractions isolated from S. typhimurium-infected cells were compared with those of mock-infected cells, revealing significant depletion or enrichment of 105 proteins. Proteins annotated to play a role in membrane traffic were overrepresented among the depleted proteins whereas proteins annotated to the cytoskeleton showed a diverse behavior with some proteins being enriched, others being depleted from the Golgi fraction upon Salmonella infection. To study the functional relevance of identified proteins in the Salmonella infection cycle, small interfering RNA (siRNA) experiments were performed. siRNA-mediated depletion of a selection of affected proteins identified five host factors involved in Salmonella infection. Depletion of peroxiredoxin-6 (PRDX6), isoform beta-4c of integrin beta-4 (ITGB4), isoform 1 of protein lap2 (erbin interacting protein; ERBB2IP), stomatin (STOM) or TBC domain containing protein 10b (TBC1D10B) resulted in increased Salmonella replication. Surprisingly, in addition to the effect on Salmonella replication, depletion of STOM or ITGB4 resulted in a dispersal of intracellular Salmonella microcolonies. It can be concluded that by using SILAC-based quantitative proteomics we were able to identify novel host cell proteins involved in the complex interplay between Salmonella and epithelial cells. Twit Text FOAVip Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle ????Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=21919203 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21919203 #FOAvip English Wikipedia <ref>{{Cite pmid|21919203}}</ref> Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle #MMPMID21919203 Vogels MW; van Balkom BW; Heck AJ; de Haan CA; Rottier PJ; Batenburg JJ; Kaloyanova DV; Helms JB Proteomics 2011[Dec]; 11 (23): 4477-91 PMID21919203show ga To identify host factors involved in Salmonella replication, SILAC-based quantitative proteomics was used to investigate the interactions of Salmonella typhimurium with the secretory pathway in human epithelial cells. Protein profiles of Golgi-enriched fractions isolated from S. typhimurium-infected cells were compared with those of mock-infected cells, revealing significant depletion or enrichment of 105 proteins. Proteins annotated to play a role in membrane traffic were overrepresented among the depleted proteins whereas proteins annotated to the cytoskeleton showed a diverse behavior with some proteins being enriched, others being depleted from the Golgi fraction upon Salmonella infection. To study the functional relevance of identified proteins in the Salmonella infection cycle, small interfering RNA (siRNA) experiments were performed. siRNA-mediated depletion of a selection of affected proteins identified five host factors involved in Salmonella infection. Depletion of peroxiredoxin-6 (PRDX6), isoform beta-4c of integrin beta-4 (ITGB4), isoform 1 of protein lap2 (erbin interacting protein; ERBB2IP), stomatin (STOM) or TBC domain containing protein 10b (TBC1D10B) resulted in increased Salmonella replication. Surprisingly, in addition to the effect on Salmonella replication, depletion of STOM or ITGB4 resulted in a dispersal of intracellular Salmonella microcolonies. It can be concluded that by using SILAC-based quantitative proteomics we were able to identify novel host cell proteins involved in the complex interplay between Salmonella and epithelial cells. |*Host-Pathogen Interactions[MESH] |Epithelial Cells/metabolism/*microbiology[MESH] |Golgi Apparatus/metabolism/microbiology[MESH] |HeLa Cells[MESH] |Humans[MESH] |Integrin beta4/genetics/metabolism[MESH] |Membrane Proteins/genetics/metabolism[MESH] |Proteome/genetics/*metabolism[MESH] |RNA, Small Interfering/genetics[MESH] |Salmonella Infections/genetics/*metabolism[MESH]Quantitative proteomic identification of host factors involved in the (epmc).pdf DeepDyve Pubget Overpricing