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10.1016/j.cmet.2011.07.009 http://scihub22266oqcxt.onion/10.1016/j.cmet.2011.07.009 21907141!3172576!21907141     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Metab 2011 ; 14 (3): 352-64 Nephropedia Template TP {{tp|p=21907141|t=2011. A SPAK isoform switch modulates renal salt transport and blood pressure |pdf=|usr=}}{{21907141}} gab.com Text A SPAK isoform switch modulates renal salt transport and blood pressure JO: Cell Metab http://www.kidney.de/pget.php?&tw=1&pmid=21907141 #FOAvip The renal thick ascending limb (TAL) and distal convoluted tubule (DCT) play central roles in salt homeostasis and blood pressure regulation. An emerging model suggests that bumetanide- and thiazide-sensitive NaCl transporters (NKCC2 and NCC) along these segments are phosphorylated and activated by WNK kinases, via SPAK and OSR1. Here, we show that a kidney-specific SPAK isoform, which lacks the kinase domain, inhibits phosphorylation of NCC and NKCC2 by full-length SPAK in vitro. Kidney-specific SPAK is highly expressed along the TAL, whereas full-length SPAK is more highly expressed along the DCT. As predicted from the differential expression, SPAK knockout in animals has divergent effects along TAL and DCT, with increased phosphorylated NKCC2 along TAL and decreased phosphorylated NCC along DCT. In mice, extracellular fluid volume depletion shifts SPAK isoform abundance to favor NaCl retention along both segments, indicating that a SPAK isoform switch modulates sodium avidity along the distal nephron. Twit Text FOAVip A SPAK isoform switch modulates renal salt transport and blood pressure ????Cell Metab http://www.kidney.de/pget.php?&tw=1&pmid=21907141 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21907141 #FOAvip English Wikipedia <ref>{{Cite pmid|21907141}}</ref> A SPAK isoform switch modulates renal salt transport and blood pressure #MMPMID21907141 McCormick JA; Mutig K; Nelson JH; Saritas T; Hoorn EJ; Yang CL; Rogers S; Curry J; Delpire E; Bachmann S; Ellison DH Cell Metab 2011[Sep]; 14 (3): 352-64 PMID21907141show ga The renal thick ascending limb (TAL) and distal convoluted tubule (DCT) play central roles in salt homeostasis and blood pressure regulation. An emerging model suggests that bumetanide- and thiazide-sensitive NaCl transporters (NKCC2 and NCC) along these segments are phosphorylated and activated by WNK kinases, via SPAK and OSR1. Here, we show that a kidney-specific SPAK isoform, which lacks the kinase domain, inhibits phosphorylation of NCC and NKCC2 by full-length SPAK in vitro. Kidney-specific SPAK is highly expressed along the TAL, whereas full-length SPAK is more highly expressed along the DCT. As predicted from the differential expression, SPAK knockout in animals has divergent effects along TAL and DCT, with increased phosphorylated NKCC2 along TAL and decreased phosphorylated NCC along DCT. In mice, extracellular fluid volume depletion shifts SPAK isoform abundance to favor NaCl retention along both segments, indicating that a SPAK isoform switch modulates sodium avidity along the distal nephron. |*Isoenzymes/deficiency/genetics/pharmacology[MESH] |*Protein Serine-Threonine Kinases/deficiency/genetics/metabolism/pharmacology[MESH] |Animals[MESH] |Blood Pressure/*physiology[MESH] |Extracellular Fluid/*metabolism[MESH] |Gene Expression Regulation[MESH] |Homeostasis[MESH] |Kidney Tubules, Distal/cytology/*metabolism[MESH] |Loop of Henle/cytology/*metabolism[MESH] |Mice[MESH] |Mice, Knockout[MESH] |Phosphorylation/drug effects/physiology[MESH] |Protein Structure, Tertiary[MESH] |Receptors, Drug/genetics/metabolism[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Salts/metabolism[MESH] |Signal Transduction[MESH] |Sodium Chloride/*metabolism[MESH] |Sodium-Potassium-Chloride Symporters/genetics/metabolism[MESH] |Solute Carrier Family 12, Member 1[MESH] |Solute Carrier Family 12, Member 3[MESH] |Symporters/genetics/metabolism[MESH]A SPAK isoform switch modulates renal salt transport and blood pressur(epmc).pdf DeepDyve Pubget Overpricing