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10.1152/ajpcell.1990.259.5.C801 http://scihub22266oqcxt.onion/10.1152/ajpcell.1990.259.5.C801 2146883!ä!2146883 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol 1990 ; 259 (5 Pt 1): C801-18 Nephropedia Template TP {{tp|p=2146883|t=1990. Basal and stretch-augmented natriuretic peptide secretion by quiescent rat atria |pdf=|usr=}}{{2146883}} gab.com Text Basal and stretch-augmented natriuretic peptide secretion by quiescent rat atria JO: Am J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=2146883 #FOAvip Absolute rates of immunoreactive atrial natriuretic peptide (ANP) secretion were measured in vitro at 37 degrees C in noncontracting preparations of combined right and left rat atria at constant distending pressures of 0 or 5.1 mmHg in presence of 0.2 mM extracellular Ca2+ concentration [( Ca2+]o), 10 microM ryanodine, and either 1 microM saxitoxin or 10 microM tetrodotoxin. By systematic deletion of external Na+, K+, Mg2+, Cl-, or HCO3-, and reduction of [Ca2+]o, and by selective ion transport inhibitors, neither net transplasmalemmal fluxes of Na+, Cl-, HCO3-, and Mg2+ nor ryanodine-sensitive Ca2+ release from sarcoplasmic reticulum (SR) was found necessary for stretch augmentation of secretory rate (Ra). Ra 1) was near zero within 20 min when extracellular Na+ concentration = 0 and [Ca2+]o = 20 microM and within 5 min or less after preincubation with caffeine, 8-chlorophenylthioadenosine 3',5'-cyclic monophosphate, or at 18 degrees C; 2) was significantly decreased by Cd2+, Ni2+, the isoquinoline H-7, and trifluoperazine but not 100 microM ryanodine; 3) was increased by neomycin; and 4) had an apparent activation energy of 18.5 +/- 4.1 x 10(3) cal/mol between 23 and 42 degrees C. These experiments strongly implicated transplasmalemmal Ca2+ influx and cAMP but not SR Ca2+ release in control of Ra under the experimental conditions studied. Twit Text FOAVip Basal and stretch-augmented natriuretic peptide secretion by quiescent rat atria ????Am J Physiol http://www.kidney.de/pget.php?&tw=1&pmid=2146883 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=2146883 #FOAvip English Wikipedia <ref>{{Cite pmid|2146883}}</ref> Basal and stretch-augmented natriuretic peptide secretion by quiescent rat atria #MMPMID2146883 Page E; Goings GE; Power B; Upshaw-Earley J Am J Physiol 1990[Nov]; 259 (5 Pt 1): C801-18 PMID2146883show ga Absolute rates of immunoreactive atrial natriuretic peptide (ANP) secretion were measured in vitro at 37 degrees C in noncontracting preparations of combined right and left rat atria at constant distending pressures of 0 or 5.1 mmHg in presence of 0.2 mM extracellular Ca2+ concentration [( Ca2+]o), 10 microM ryanodine, and either 1 microM saxitoxin or 10 microM tetrodotoxin. By systematic deletion of external Na+, K+, Mg2+, Cl-, or HCO3-, and reduction of [Ca2+]o, and by selective ion transport inhibitors, neither net transplasmalemmal fluxes of Na+, Cl-, HCO3-, and Mg2+ nor ryanodine-sensitive Ca2+ release from sarcoplasmic reticulum (SR) was found necessary for stretch augmentation of secretory rate (Ra). Ra 1) was near zero within 20 min when extracellular Na+ concentration = 0 and [Ca2+]o = 20 microM and within 5 min or less after preincubation with caffeine, 8-chlorophenylthioadenosine 3',5'-cyclic monophosphate, or at 18 degrees C; 2) was significantly decreased by Cd2+, Ni2+, the isoquinoline H-7, and trifluoperazine but not 100 microM ryanodine; 3) was increased by neomycin; and 4) had an apparent activation energy of 18.5 +/- 4.1 x 10(3) cal/mol between 23 and 42 degrees C. These experiments strongly implicated transplasmalemmal Ca2+ influx and cAMP but not SR Ca2+ release in control of Ra under the experimental conditions studied. |Amiloride/pharmacology[MESH] |Animals[MESH] |Atrial Function[MESH] |Atrial Natriuretic Factor/*metabolism[MESH] |Cadmium/pharmacology[MESH] |Calcium Channel Blockers/pharmacology[MESH] |Female[MESH] |Heart Atria/drug effects/ultrastructure[MESH] |Heart/*physiology[MESH] |In Vitro Techniques[MESH] |Kinetics[MESH] |Microscopy, Electron[MESH] |Myocardial Contraction[MESH] |Rats[MESH] |Rats, Inbred Strains[MESH] |Ryanodine/pharmacology[MESH] |Saxitoxin/pharmacology[MESH] |Tetrodotoxin/pharmacology[MESH]Basal and stretch-augmented natriuretic peptide secretion by quiescent(epmc).pdf DeepDyve Pubget Overpricing