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10.1002/ppul.21385 http://scihub22266oqcxt.onion/10.1002/ppul.21385 21194137!7167622!21194137     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Pediatr+Pulmonol 2011 ; 46 (5): 428-34 Nephropedia Template TP {{tp|p=21194137|t=2011. Impact of PCR for respiratory viruses on antibiotic use: theory and practice |pdf=|usr=}}{{21194137}} gab.com Text Impact of PCR for respiratory viruses on antibiotic use: theory and practice JO: Pediatr Pulmonol http://www.kidney.de/pget.php?&tw=1&pmid=21194137 #FOAvip RATIONALE FOR THE STUDY: Real-time polymerase chain reaction (PCR) for respiratory viruses is more sensitive, yet more expensive, than conventionally used direct immunofluorescence (DIF). We determined the impact of real-time PCR, additional to DIF, on antibiotic prescription in ventilated children with lower respiratory tract infection (LRTI) at admission to the pediatric intensive care unit (PICU). METHODS: First, a multicenter survey study was performed. Subsequently, in a prospective study, children (</= 5 years) with LRTI were tested at admission by DIF and PCR. Positive DIF results were reported at the end of the first working day. PICU physicians reported antibiotic treatment on the second working day. After informing them of the PCR result antibiotic treatment was reevaluated. RESULTS: The multicenter survey study (94 respondents) showed that PCR decreased antibiotic use (P < 0.001). In the prospective study 38 children were included, of which 19 (50%) were DIF positive. Of the 19 DIF negative patients 12 (63%) were treated with antibiotics before revealing the PCR result; the PCR test was positive in 9 out of 12. Revealing PCR results did not alter antibiotic treatment. In 7 DIF negative patients antibiotics not given, the PCR test was positive. CONCLUSION: In contrast to their responses to the survey study, in real-life PICU physicians did not let their antibiotic prescription be influenced by respiratory real-time PCR in children ventilated for LRTI. Twit Text FOAVip Impact of PCR for respiratory viruses on antibiotic use: theory and practice ????Pediatr Pulmonol http://www.kidney.de/pget.php?&tw=1&pmid=21194137 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21194137 #FOAvip English Wikipedia <ref>{{Cite pmid|21194137}}</ref> Impact of PCR for respiratory viruses on antibiotic use: theory and practice #MMPMID21194137 van de Pol AC; Wolfs TF; Tacke CE; Uiterwaal CS; Forster J; van Loon AM; Kimpen JL; Rossen JW; Jansen NJ Pediatr Pulmonol 2011[May]; 46 (5): 428-34 PMID21194137show ga RATIONALE FOR THE STUDY: Real-time polymerase chain reaction (PCR) for respiratory viruses is more sensitive, yet more expensive, than conventionally used direct immunofluorescence (DIF). We determined the impact of real-time PCR, additional to DIF, on antibiotic prescription in ventilated children with lower respiratory tract infection (LRTI) at admission to the pediatric intensive care unit (PICU). METHODS: First, a multicenter survey study was performed. Subsequently, in a prospective study, children ( |Anti-Bacterial Agents/*therapeutic use[MESH] |Child, Preschool[MESH] |DNA, Viral/*analysis[MESH] |Female[MESH] |Hospitalization[MESH] |Humans[MESH] |Infant[MESH] |Infant, Newborn[MESH] |Intensive Care Units, Pediatric[MESH] |Male[MESH] |Practice Patterns, Physicians'[MESH] |Prospective Studies[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Respiratory Tract Infections/*drug therapy/virology[MESH]Impact of PCR for respiratory viruses on antibiotic use: theory and pr(epmc).pdf DeepDyve Pubget Overpricing