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10.1002/pmic.201000309 http://scihub22266oqcxt.onion/10.1002/pmic.201000309 21182195!7167679!21182195     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proteomics 2011 ; 11 (1): 64-80 Nephropedia Template TP {{tp|p=21182195|t=2011. Identification of host factors involved in coronavirus replication by quantitative proteomics analysis |pdf=|usr=}}{{21182195}} gab.com Text Identification of host factors involved in coronavirus replication by quantitative proteomics analysis JO: Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=21182195 #FOAvip In this study, we applied a quantitative proteomic approach, based on SILAC, to investigate the interactions of coronaviruses with the secretory pathway of the host cell, with the aim to identify host factors involved in coronavirus replication. Comparison of the protein profiles of Golgi-enriched fractions of cells that were either mock infected or infected with mouse hepatitis virus revealed the significant depletion or enrichment of 116 proteins. Although ribosomal/nucleic acid binding proteins were enriched in the Golgi-fractions of mouse hepatitis virus-infected cells, proteins annotated to localize to several organelles of the secretory pathway were overrepresented among the proteins that were depleted from these fractions upon infection. We hypothesized that proteins, of which the abundance or distribution is affected by infection, are likely to be involved in the virus life cycle. Indeed, depletion of a small subset of the affected proteins by using small interfering RNAs identified several host factors involved in coronavirus infection. Transfection of small interfering RNAs targeting either C11orf59 or Golgi apparatus glycoprotein 1 resulted in increased virus replication, whereas depletion of vesicle-trafficking protein vesicle-trafficking protein sec22b enhanced the release of infectious progeny virus. Overexpression of these proteins, on the other hand, had a negative effect on virus replication. Overall, our study shows that the SILAC approach is a suitable tool to study host-pathogen interactions and to identify host proteins involved in virus replication. Twit Text FOAVip Identification of host factors involved in coronavirus replication by quantitative proteomics analysis ????Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=21182195 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21182195 #FOAvip English Wikipedia <ref>{{Cite pmid|21182195}}</ref> Identification of host factors involved in coronavirus replication by quantitative proteomics analysis #MMPMID21182195 Vogels MW; van Balkom BW; Kaloyanova DV; Batenburg JJ; Heck AJ; Helms JB; Rottier PJ; de Haan CA Proteomics 2011[Jan]; 11 (1): 64-80 PMID21182195show ga In this study, we applied a quantitative proteomic approach, based on SILAC, to investigate the interactions of coronaviruses with the secretory pathway of the host cell, with the aim to identify host factors involved in coronavirus replication. Comparison of the protein profiles of Golgi-enriched fractions of cells that were either mock infected or infected with mouse hepatitis virus revealed the significant depletion or enrichment of 116 proteins. Although ribosomal/nucleic acid binding proteins were enriched in the Golgi-fractions of mouse hepatitis virus-infected cells, proteins annotated to localize to several organelles of the secretory pathway were overrepresented among the proteins that were depleted from these fractions upon infection. We hypothesized that proteins, of which the abundance or distribution is affected by infection, are likely to be involved in the virus life cycle. Indeed, depletion of a small subset of the affected proteins by using small interfering RNAs identified several host factors involved in coronavirus infection. Transfection of small interfering RNAs targeting either C11orf59 or Golgi apparatus glycoprotein 1 resulted in increased virus replication, whereas depletion of vesicle-trafficking protein vesicle-trafficking protein sec22b enhanced the release of infectious progeny virus. Overexpression of these proteins, on the other hand, had a negative effect on virus replication. Overall, our study shows that the SILAC approach is a suitable tool to study host-pathogen interactions and to identify host proteins involved in virus replication. |Coronavirus/*physiology[MESH] |Electrophoresis, Polyacrylamide Gel[MESH] |HeLa Cells[MESH] |Host-Pathogen Interactions/genetics/*physiology[MESH] |Humans[MESH] |Mass Spectrometry[MESH] |Murine hepatitis virus/physiology[MESH] |Proteomics/*methods[MESH] |RNA Interference[MESH] |RNA, Small Interfering[MESH] |Receptors, Fibroblast Growth Factor/genetics/metabolism[MESH] |Sialoglycoproteins/genetics/metabolism[MESH]Identification of host factors involved in coronavirus replication by (epmc).pdf DeepDyve Pubget Overpricing